绵羊DFAT细胞特性及其诱导成脂分化过程中关键基因的表达  

Properties of Sheep DFAT Cells and Expression of Key Genes in Induced Adipogenic Differentiation

作　　者：王世银[1] 郭庆河[1] 宁程程 高莉[1] 牛嘉 吕刚 张伟[1] 王全得[4] WANG Shiyin;GUO Qinghe;NING Chengcheng;GAO Li;NIU Jia;L Gang;ZHANG Wei;WANG Quande(Xinjiang Agricultural Vocational Technical College,Key Laboratory of Livestock and Poultry Healthy Breeding Technology in Northwest China,Ministry of Agriculture and Rural Affairs,Changji Xinjiang 831100,China;Western Research Institute,Chinese Academy of Agricultural Science,Changji Xinjiang 831100,China;Xinjiang Taikun Group Co.,Ltd.,Changji Xinjiang 831100,China;Agricultural Development Service Center of Regiment 103,6th Division,Xinjiang Production and Construction Corps,Wujiaqu Xinjiang 831300,China)

机构地区：[1]新疆农业职业技术学院,农业农村部西北畜禽健康养殖技术重点实验室,新疆昌吉831100 [2]中国农业科学院西部农业研究中心,新疆昌吉831100 [3]新疆泰昆集团有限责任公司,新疆昌吉831100 [4]新疆生产建设兵团第六师103团农业发展服务中心,新疆五家渠831300

出　　处：《西北农业学报》2025年第3期387-396,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：新疆维吾尔自治区自然科学基金(2022D01A92,2022D01E18);新疆现代农业产业技术体系专项经费(XJARS-09)。

摘　　要：为了揭示绵羊DFAT细胞特性及其诱导成脂分化过程中关键基因的表达,以实验室前期获得的不同年龄阿勒泰羊尾部脂肪组织DFAT细胞为试验材料,对其细胞增殖能力、诱导成脂分化能力及其诱导成脂分化过程中关键基因的表达规律进行深入研究。结果表明:来源于6、12和24月龄阿勒泰羊尾部脂肪组织的第3代DFAT细胞生长曲线均呈典型的“S”形曲线,细胞增殖速度未见明显差异;细胞连续传代20代后仍保持良好的细胞形态和增殖速度,且不同代次细胞之间未见明显差异;采用诱导和维持培养交替进行的诱导成脂分化体系(体系Ⅲ),其诱导成脂分化效率极显著优于持续诱导分化体系(体系Ⅰ)和先诱导然后维持培养的诱导分化体系(体系Ⅱ)(P<0.01);不同年龄来源DFAT细胞诱导成脂分化能力差异不显著(P>0.05);在DFAT细胞诱导成脂分化过程中,启动成脂分化的关键基因PPARγ和C/EBPα,脂质沉积关键基因ADIPOQ、FSP27、FABP4和LPL,脂质分解关键基因HSL、ATGL和Leptin,以及前体脂肪细胞标志性基因CD142和ICAM1均先出现极显著上调(P<0.01),然后又出现极显著下调(P<0.01),但脂肪细胞祖细胞标志性基因DPP4的表达量在诱导分化的第2天以后出现极显著下调(P<0.01),且在此后的整个诱导分化期间均保持极低的表达水平。研究结果证明在阿勒泰羊生命周期的很长时间内,其DFAT细胞均保持了良好的增殖及成脂分化能力。To elucidate the characteristics of sheep DFAT cells and the expression of key genes during adipogenic differentiation,DFAT cells from tail fat tissue of Altay sheep at different ages were used as experimental materials.This study investigated their cell proliferation ability,adipogenic differentiation potential,and expression patterns of key genes during adipogenic differentiation.The results showed that the growth curves of the 3rd generation DFAT cells from the adipose tissue of Altay sheep at 6,12 and 24 months old showed typical“S”shapes,with no significant differences in cell proliferation rate.The cell morphology and proliferation rate remained stable after 20 generations,with no significant differences among different generations of cells.The efficiency of induced lipid differentiation system III,alternating induction and maintenance culture,was significantly superior to both system I(continuous induction)and system II(initial induction followed by maintenance culture)(P<0.01).There was no significant difference in the adipogenic differentiation ability of DFAT cells from different ages(P>0.05).During induced adipogenic differentiation of DFAT cells,the key adipogenic differentiation genes PPARγand C/EPBα,lipid deposition genes ADIPOQ,FSP27,FABP4 and LPL,lipid decomposition genes HSL,ATGL and Leptin,and the preadipocytes signature genes CD142 and ICAM1 were significantly up-regulated first(P<0.01)and then significantly down-regulated(P<0.01).However,the expression of the adipocyte progenitor cells signature gene DPP4 was significantly down-regulated during the next day of induced differentiation(P<0.01)and remained extremely low expression levels throughout the induced differentiation period.This study demonstrates that DFAT cells of Altay sheep retain robust proliferation and adipogenic differentiation capabilities over a long period.The findings lay a foundation for further research on the biological functions of sheep adipocytes and breeding of lean meat sheep breeds.

关 键 词：绵羊 阿勒泰羊 DFAT细胞 诱导 成脂分化 脂肪细胞 

分 类 号：R73[医药卫生—肿瘤]