Expression ofγδT cells and lymphocyte subsets in newly diagnosed multiple myeloma patients  

作　　者：Juan Huang Ping-ting Pu Xu Wang Rui-xin Sun Xiang-yi Zhao Ling-yuan Feng Zi-zhen Xu 

机构地区：[1]Department of Laboratory Medicine,College of Health Science and Technology,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China [2]Department of Clinical Laboratory,Yangpu Hospital Affiliated to Tongji University,Shanghai 200090,China

出　　处：《LabMed Discovery》2024年第1期49-55,共7页检验医学发现(英文)

基　　金：supported by the National Natural Science Foundation of China(Nos.81600151,81302039 and 82060040).

摘　　要：Objective:To retrospectively analyze the expression and significance ofγδT cells and lymphocyte subsets in peripheral blood on patients with multiple myeloma(MM).Methods:Fifty-five patients with multiple myeloma admitted to Yangpu Hospital Affiliated to Tongji University from January 2022 to August 2023 were selected as the disease group,and were categorized into stage I group,stage II group and stage III group according to the International Staging System(ISS)of multiple myeloma.All included patients were consecutive visits.Another 30 cases of healthy people with physical examination were selected as the control group.Flow cytometry was used to detect theγδT cells and lymphocyte subsets in the peripheral blood of the subjects.After clinical indicators of patients and healthy controls were collected,the statistical analysis was conducted.Results:Compared with the control group,the percentage ofγδT cells and CD8^(+)T cells in the disease group were higher,while,the absolute numbers of total peripheral blood lymphocytes(LYMT)and CD19^(+)B cells,percentage of CD19^(+)B cells,CD3^(+)T cells,CD4^(+)T cells were lower(P<0.05).The percentage ofγδT cells(P<0.001),the percentage of CD19^(+)B cells(P=0.003),the absolute number of CD3^(+)T cells(P=0.010),the absolute number of CD8^(+)T cells(P=0.002)were the significant influential factors of MM.γδT cells(P=0.004),neutrophils(P<0.001),neutrophil to lymphocyte ratio(P=0.003),and white blood cell count(P<0.001)were statistically different in different stages.ROC curve suggested that the area under the curve of the percentage ofγδT cells in predicting the MM were 0.973(P<0.001)which had certain diagnostic value.Conclusions:γδT cells and lymphocyte subsets(CD8^(+)T cells,CD19^(+)B cells,CD3^(+)T cells,etc.)in patients with newly diagnosis of MM can reflect the immune function of the patient’s organism,which is a key factor of MM.γδT cells influence the staging of MM patients,and also have certain diagnostic value.

关 键 词：Multiple myeloma Lymphocyte subset γδT cell Monoclonal Immunoglobulin ANEMIA HIV infection HYPERTENSION 

分 类 号：R73[医药卫生—肿瘤]