Optimized collision energy based multiple reaction monitoring facilitated validation of immune cell activated proteins in 3D cancer-Immune cell co-culture model  

作　　者：Rajdeep Chakraborty Thiri Zaw 

机构地区：[1]Applied Biosciences,Faculty of Science and Engineering,Macquarie University,Sydney,NSW 2109,Australia [2]School of Natural Sciences,Faculty of Science and Engineering,Macquarie University,Sydney,NSW 2109,Australia [3]Australian Proteome Analysis Facility,Faculty of Science and Engineering,Macquarie University,Sydney,NSW 2109,Australia

出　　处：《LabMed Discovery》2024年第2期89-94,共6页检验医学发现(英文)

基　　金：supported by Australian and New Zealand Head and Neck Cancer Society Trudi Shine Adenoid Cystic Carcinoma Grant Award 2021.

摘　　要：Objective:Multiple reaction monitoring(MRM),is a highly sensitive targeted mass spectrometry technique that is used to validate discovery phase mass spectrometry data.Through this simple technique,relative protein expressions can be acquired without using commercially available antibodies.Methods:Our previous work showed Gipie silencing of UM-HACC-2A(adenoid cystic carcinoma)cells resulted in upregulation of a plethora of immune proteins in immune cells in 3D ACC-immune co-culture model.Results:MRM enabled determining relative protein expression of significantly upregulated immune proteins due to silencing of Gipie.Granzyme A,CD48,granzyme B,HLA class I,antigen B,HLA class I,antigen A,galectin 1,vimentin,endoplasmic reticulum chaperone BiP,and dipeptidyl peptidase 1 differential expressions were validated.Whereas coactosin-like protein expressions were found non-significant among control and Gipie silenced ACC.Conclusions:This manuscript presents the validation data of previous discovery phase mass spectrometry and also accentuate the use of MRM in translational or preclinical studies in the field of biomedical sciences.

关 键 词：Multiple reaction monitoring Biomolecular detection Salivary gland cancer Immune evasion Adenoid cystic carcinoma 

分 类 号：R73[医药卫生—肿瘤]