免疫组化PTEN蛋白与MMR蛋白在子宫内膜样癌中的表达及其相关性研究  

Expression and Correlation of PTEN and MMR in Endometrioid Carcinoma by Immunohistochemistry

作　　者：肖诗维 孙文佳 金苏 岳君秋 郭芳 XIAO Shiwei;SUN Wenjia;JIN Su(Department of Pathology,Hubei Cancer Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan Hubei 430079,China)

机构地区：[1]华中科技大学同济医学院附属湖北肿瘤医院病理科,湖北武汉430079

出　　处：《实用妇产科杂志》2025年第2期143-149,共7页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:探讨子宫内膜样癌(EEC)中第10号染色体缺失的磷酸酶及张力蛋白同源物(PTEN)蛋白的免疫组织化学表达模式及其与DNA错配修复(MMR)蛋白表达的关系。方法:选择2019年5月14日至2023年6月7日于华中科技大学同济医学院附属湖北肿瘤医院病理科确诊的EEC、子宫内膜非典型增生/子宫内膜上皮内瘤变(EAH/EIN)及因子宫肌瘤切除子宫的正常子宫内膜共121例患者为研究对象,其中EEC组84例、EAH/EIN组17例、对照组20例。采用免疫组织化学检测3组的子宫内膜组织中PTEN蛋白和MMR蛋白的表达模式及表达差异,并比较MMR蛋白缺失(MMRd)组和未缺失(MMRp)组EEC中PTEN蛋白异常表达的差异。结果:①PTEN蛋白在121例子宫内膜组织腺上皮细胞中的表达包括4种模式:正常表达、阴性表达、阳性表达减弱和异质性表达。②PTEN蛋白在EEC组、EAH/EIN组中异常表达率(84.5%、94.1%)高于对照组(10.0%);MMR蛋白在EEC组、EAH/EIN组中MMRd表达率(35.7%、35.3%)高于对照组(0%),以上差异均有统计学意义(P<0.05)。③MMRd组中PTEN蛋白的异常表达率高于MMRp组(96.7%vs.77.8%,P=0.048)。④EEC组中,PTEN蛋白正常表达和异常表达的情况下,MMRd组与MMRp组中不同病理分级、肌层浸润深度、淋巴结是否转移及淋巴脉管是否侵犯的比较,差异均无统计学意义(P>0.05)。结论:通过辨识PTEN蛋白的异常表达模式并联合MMR蛋白检测有助于子宫内膜异型腺体的甄别,EEC中PTEN、MMR蛋白表达之间可能存在相关性。PTEN的异常表达对于MMRd表达EEC的生物学行为可能无明确影响。Objective:To investigate the immunohistochemical expression pattern of phosphatase and tensin homolog deleted on chromosome ten(PTEN)protein with chromosome 10 deletion in endometrioid carcinoma(EEC)and its relationship with DNA mismatch repair(MMR)protein.Methods:A total of 121 patients diagnosed with EEC,endometrial atypical hyperplasia/endometrial intraepithelial neoplasia(EAH/EIN),and normal endometrium due to uterine fibroid resection at Hubei Cancer Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from May 14,2019 to June 7,2023 were selected as the study subjects,including 84 patients in the EEC group,17 patients in the EAH/EIN group,and 20 patients in the control group.Immunohistochemical was used to detect the expression patterns and differences of PTEN and MMR in endometrial tissues of three groups,and the differences in abnormal expression of PTEN protein in EEC between MMR protein deficient(MMRd)group and non-deficient(MMRp)group were compared.Results:①The expression of PTEN protein in 121 endometrial glandular epithelial cells includes four patterns:normal expression,negativeexpression,reduced expression,and heterogeneous expression.②The abnormal expression rates of PTEN protein in the EEC and EAH/EIN groups(84.5%and 94.1%)were higher than those in the control group(10.0%);The expression rates of MMRd protein in the EEC group and EAH/EIN group(35.7%and 35.3%)were higher than those in the control group(0%),and the above differences were statistically significant(P<0.05).③The abnormal expression rate of PTEN in the MMRd group was 96.7%,which was higher than that in the MMRp group(96.7%vs.77.8%,P=0.048).④In the EEC group,with normal and abnormal expression of PTEN protein,there was no statistically significant difference in the comparison of different pathological grades,muscle infiltration depth,lymph node metastasis,and lymphatic vessel invasion between the MMRd group and the MMRp group(P>0.05).Conclusions:Identifying the abnormal expression patter

关 键 词：子宫内膜样癌 第10号染色体缺失的磷酸酶及张力蛋白同源物 DNA错配修复 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]