MAGE-A3基因对卵巢癌的生物行为学及耐药性的影响  

The Effect of MAGE-A3 Gene on the Biological Behavior and Drug Resistance of Ovarian Cancer

作　　者：王如梦 张宁[1] 吉亚南 WANG Rumeng;ZHANG Ning;JI Yanan(Bayannaoer Hospital Central Laboratory,Bayannaoer Inner Mongolia 015000,China;Inner Mongolia Autonomous Region People's Hospital Gynecology,Hohhot Inner Mongolia 010059,China)

机构地区：[1]巴彦淖尔市医院中心实验室,内蒙古巴彦淖尔015000 [2]内蒙古自治区人民医院妇科,内蒙古呼和浩特010059

出　　处：《实用妇产科杂志》2025年第2期150-157,共8页Journal of Practical Obstetrics and Gynecology

基　　金：内蒙古自治区自然科学基金项目(编号:2020MS08149)。

摘　　要：目的:探究敲减及过表达黑色素瘤相关抗原-A3(MAGE-A3)基因对卵巢癌细胞生物行为学及顺铂耐药性的影响。方法:检测卵巢癌细胞中MAGE-A3 mRNA及蛋白的表达量;构建敲减细胞株分为对照组、空载(NC)组、sh MAGE-A3组,过表达细胞株分为对照组、空载(NC)组、OE-MAGE-A3组;比较敲减和过表达MAGE-A3基因各组对肿瘤细胞增殖、迁移、侵袭和凋亡能力的影响;检测p53、Survivin、乳腺癌易感基因1(BRCA1)和MAGE-A3蛋白的表达情况。将高表达细胞株分为对照组、顺铂组、sh MAGE-A3组、顺铂+sh MAGE-A3组,低表达细胞株分为对照组、顺铂组、OE-MAGE-A3组、顺铂+OE-MAGE-A3组;比较各组细胞增殖、侵袭能力及对各基因mRNA和蛋白表达水平的影响。结果:SKOV3细胞中MAGE-A3的mRNA及蛋白高表达,A2780细胞中低表达;与对照组、NC组相比,sh MAGE-A3组OD_(450)值、迁移率、侵袭个数更低,凋亡率更高,MAGE-A3、Survivin蛋白表达更低,BRCA1、p53蛋白表达更高;OE-MAGE-A3组与对照组、NC组比较结果与sh MAGE-A3组均相反。与对照组、顺铂组、sh-MAGE-A3组比较,顺铂+sh-MAGE-A3组细胞OD_(450)值、侵袭个数更低,p53、BRCA1 mRNA和蛋白表达水平更高,Survivin mRNA和蛋白表达水平更低。顺铂+OE-MAGE-A3组细胞OD_(450)值、侵袭个数高于顺铂组,低于对照组、OE-MAGE-A3组;p53、BRCA1 mRNA和蛋白表达水平高于对照组、OE-MAGE-A3组,低于顺铂组;Survivin mRNA和蛋白表达水平低于对照组、OE-MAGE-A3组,高于顺铂组。结论:敲低MAGE-A3表达可有效抑制卵巢癌细胞的增殖、迁移和侵袭,促进细胞凋亡,增强顺铂敏感性。相反,过表达MAGE-A3增加细胞增殖、迁移和侵袭,减少细胞凋亡,从而赋予顺铂耐药性。Objective:To investigate the effects of knockdown and overexpression of melanoma-associated antigen-A3(MAGE-A3)gene on the biological behavior and cisplatin resistance of ovarian cancer cells.Methods:The expression level of MAGE-A3 mRNA and protein in ovarian cancer cells was detected.The knockdown cell lines were constructed and divided into control group,no-load(NC)group and sh MAGE-A3 group.The overexpression cell lines were divided into control group,no-load(NC)group and OE-MAGE-A3 group.The effects of knockdown and overexpression of MAGE-A3 gene on the proliferation,migration,invasion and apoptosis of tumor cells were compared.The expression of p53,Survivin,breast cancer susceptibility gene 1(BRCA1)and MAGE-A3 protein was detected.The high expression cell lines were divided into control group,cisplatin group,shMAGE-A3 group and cisplatin+shMAGE-A3 group.The low expression cell lines were divided into control group,cisplatin group,OE-MAGE-A3 group and cisplatin+OE-MAGE-A3 group.The cell proliferation,invasion ability of each group of cells,as well as their effects on the mRNA and protein expression level of each gene were compared.Results:The mRNA and protein expression of MAGE-A3 was high in SKOV3 cells and low in A2780 cells.Compared with the control group and NC group,the shMAGE-A3 group had lower OD_(450)value,migration rate and invasion numbers,higher apoptosis rate,lower expression of MAGE-A3 and Survivin protein,and higher expression of BRCA1 and p53 protein.The results of OE-MAGE-A3 group compared with control group and NC group were opposite to those of shMAGE-A3 group.Compared with the control group,cisplatin group and sh-MAGE-A3 group,the cisplatin+sh-MAGE-A3 group had lower OD_(450)value and invasion number higher levels of p53 and BRCA1 mRNA and protein expression,and lower levels of Survivin mRNA and protein.The OD_(450)value and invasion number of cells in cisplatin+OE-MAGE-A3 group were higher than those in cisplatin group,but lower than those in control group and OE-MAGE-A3 group.The mRNA and

关 键 词：卵巢癌 黑色素瘤相关抗原-A3 侵袭 迁移 凋亡 耐药性 

分 类 号：R737.31[医药卫生—肿瘤]