一株高效降解饲料中呕吐毒素菌株的分类鉴定和作用条件优化研究  

Identification and condition optimization of an effective deoxynivalenol-degradation strain JM-3 in feeds

作　　者：赵相艳 黄海 郭宏伟[1] 郑鸣[1] 赵文巧 李京 张震[1] 潘春梅[1] ZHAO Xiangyan;HUANG Hai;GUO Hongwei;ZHENG Ming;ZHAO Wenqiao;LI Jing;ZHANG Zhen;PAN Chunmei(College of Food and Bioengineering,Henan University of Animal Husbandry and Economy,Zhengzhou,Henan Province 450046,China;Sinagri YingTai Bio-Peptide Co.Ltd,Anyang,Henan Province 456550,China)

机构地区：[1]河南牧业经济学院食品与生物工程学院,河南郑州450046 [2]中农颖泰林州生物科园有限公司,河南安阳456550

出　　处：《中国饲料》2025年第5期30-35,共6页China Feed

基　　金：河南省重点研发专项(231111112000,241111113400);河南省科技攻关项目(222102110130,222102110158,222102310229,242102111035);河南牧业经济学院博士科研启动项目(M4050008);河南牧业经济学院食品科学与工程重点学科项目(XJXK202203)。

摘　　要：呕吐毒素(DON)广泛存在于自然界,容易污染谷物和饲料,人和动物一旦摄入被污染的谷物和饲料将会对健康造成严重威胁,因此DON的脱毒研究迫在眉睫。本研究针对前期筛选分离得到的一株对低浓度DON有高效降解能力的菌株JM-3,通过形态学显微观察、生理生化反应、全基因组测序和系统发育进化树的构建对其进行分类鉴定;通过绘制其生长曲线了解其生长特征;通过高效液谱法(HPLC)检测JM-3菌株在不同温度、pH和作用时间下对DON的降解效率。结果显示:该菌株的最佳作用条件为:温度30℃、pH 7.0,在48 h内可将DON降解97.8%。该试验为研制德沃斯氏菌JM-3菌株的微生态制剂及进一步应用奠定了基础。Vomitoxin is widely distributed in nature and easily contaminates grains and feed.Once humans and animals ingests contaminated grains and feed,it will pose a serious threat to their health.In this study,a strain JM-3 was isolated before,which was with high efficiency in degrading low concentrations of deoxynivalenol(DON).Classification and identification were performed by screening and identified by morphological microscopic observation,physiological and biochemical reactions,whole genome sequencing,and phylogenetic tree construction;Its growth characteristics were analyzed by drawing its growth curve.The degradation efficiency of DON by JM-3 strain at different temperatures,pH and reaction time were detected by high performance liquid chromatograph(HPLC),and then the optimal action conditions of JM-3 strain were obtained.The results showed that the JM-3 strain was identified as Devosia Sp.,and its optimum reaction conditions were:temperature 30℃,pH 7.0.Under such reaction condition,97.8%of the DON could be degraded within 48 h.Thereafter,the degradation rate of DON was maintained at this level with the time prolonging,which would lay a foundation for the development of microecological agents and further application of the JM-3 strain.

关 键 词：呕吐毒素 HPLC 德沃斯氏菌 作用条件 

分 类 号：S816.3[农业科学—饲料科学]