Gut microbial characteristics of the damp-heat constitution:a population-based multicenter cross-sectional study  

作　　者：LI Tianxing ZHU Linghui WANG Xueke TANG Jun YANG Lingling PANG Guoming LI Huang WANG Liying DONG Yang ZHAO Shipeng LI Yingshuai LI Lingru 

机构地区：[1]Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China [2]National Institute of TCM Constitution and Preventive Medicine,Beijing University of Chinese Medicine,Beijing 100029,China [3]the Second Clinical Medical College,Henan University of Chinese Medicine,Zhengzhou 450046,China [4]Department of Hepatobiliary Spleen and Stomach,the First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China [5]Henna Province Hospital of Traditional Chinese Medicine,Henan 450002,China [6]Department of Endocrinology,Kaifeng Hospital of traditional Chinese Medicine,Kaifeng 475001,China [7]State Key Laboratory of Dampness Syndrome of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China [8]College of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China [9]Graduate School of China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Journal of Traditional Chinese Medicine》2025年第1期140-151,共12页中医杂志(英文版)

基　　金：National Nonprofit Institute Research Grant for the Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medical Sciences:Mechanism of Regulating Phlegm-Dampness Constitution to Prevent Metabolic Diseases based on Gut Microbiota-host DNA Methylation(No.YZ-202151)。

摘　　要：OBJECTIVE:To investigate the difference in gut microbiota between population with damp-heat constitution(DHC)and balanced constitution(BC).METHODS:A multi-centered cross-sectional casecontrol study was conducted,which included 249 participants with damp-heat constitution or balanced constitution.Baseline information of participants was collected,and stool samples were collected for gut microbiota analysis.Principal coordinate analysis,linear discriminant analysis effect size analysis,receiver operating characteristic,random forest model,and phylogenetic investigation of communities by reconstruction of unobserved states methods were used to reveal the relationship between gut microbiota and the damp-heat constitution.RESULTS:Compared to those in the BC group,the richness and diversity of the microbiota,specifically those of several short-chain fatty acid producing genera such as Barnesiella,Coprobacter,and Butyricimonas,were significantly decreased in the DHC group.Regarding biological functions,flavonoid biosynthesis,propanoate metabolism,and nucleotide sugar metabolism were suppressed,while arachidonic acid metabolism and glutathione metabolism were enriched in the DHC group.Finally,a classifier based on the microbiota was constructed to discriminate between the DHC and BC populations.CONCLUSION:The gut microbiota of the DHC population exhibits significantly reduced diversity and is closely related to inflammation,metabolic disorders,and liver steatosis,which is consistent with clinical observations,thus serving as a potential diagnostic tool for traditional Chinese medicine constitution discrimination.

关 键 词：damp-heat constitution balanced constitution Traditional Chinese medicine constitution MICROBIOTA inflammatory PICRUSt multicenter study cross-sectional studies 

分 类 号：R229[医药卫生—中医基础理论]