电热针对膝骨关节炎大鼠病理性疼痛、内质网应激及EGFR/JAK/STAT信号通路的影响  

Effects of electroheating needle on pathologic pain,endoplasmic reticulum stress and EGFR/JAK/STAT signaling pathway in rats with knee osteoarthritis

作　　者：张杰 许明军 杨远征 Zhang Jie;Xu Mingjun;Yang Yuanzheng(Department of Acupuncture Treatment,Shiyan Taihe Hospital,Affiliated Hospital of Hubei Medical College,Shiyan 442000,China;Outpatient Rehabilitation Therapy Unit,Shiyan Taihe Hospital,Affiliated Hospital of Hubei Medical College,Shiyan 442000,China)

机构地区：[1]十堰市太和医院/湖北医药学院附属医院针灸科,十堰442000 [2]十堰市太和医院/湖北医药学院附属医院门诊康复治疗科,十堰442000

出　　处：《国际中医中药杂志》2025年第3期342-347,共6页International Journal of Traditional Chinese Medicine

基　　金：湖北省中医药管理局中医药科研项目(ZY2023F072);十堰市太和医院院级课题项目(2024JJXM071)。

摘　　要：目的探究电热针对膝骨关节炎模型大鼠病理性疼痛、内质网应激及EGFR/Janus激酶(JAK1)/STAT1信号通路的影响。方法将50只SPF级SD雄性大鼠按随机数字表法分为正常组10只和造模组40只。造模组采用关节腔注射碘乙酸钠法制备膝骨关节炎模型。将造模成功大鼠分为模型组、透明质酸钠组、电热针组,每组10只。透明质酸钠组大鼠关节腔注射透明质酸钠0.1 ml,电热针组给予电热针干预,正常组、模型组大鼠灌胃等体积生理盐水,1次/d,干预2周。检测大鼠机械痛阈值(PWT)和热痛阈值(PWL),HE染色检测软骨组织病理形态,免疫组化染色检测软骨组织葡萄糖调节蛋白78(GRP78)、C/EBP同源蛋白(CHOP)表达,Western blot法检测EGFR、JAK、STAT蛋白表达,RT-PCR法检测软骨组织GRP78、CHOP、EGFR、JAK、STAT mRNA水平。结果正常组大鼠软骨组织结构正常,滑膜细胞分布有序,软骨细胞排列整齐,未见炎性细胞浸润;模型组滑膜增生肥厚,滑膜细胞排列不规则且大量增生,软骨细胞排列紊乱且数目减少,可见大量炎性细胞浸润。与模型组比较,透明质酸钠组、电热针组PWT、PWL升高(P<0.05),软骨组织GRP78、CHOP表达降低(P<0.05),EGFR、JAK1、STAT1蛋白表达降低(P<0.05),GRP78、CHOP、EGFR、JAK1、STAT1 mRNA水平降低(P<0.05)。结论电热针可改善膝骨关节炎模型大鼠病理性疼痛,其作用机制可能与抑制内质网应激,调控EGFR/JAK/STAT信号通路有关。Objective To investigate the effects of electroheating on pathologic pain,endoplasmic reticulum stress and EGFR/JAK/STAT signaling pathway in rats with knee osteoarthritis.Methods Totally 40 SPF SD male rats were divided into normal group(10 rats)and modeling group(40 rats)with random number table method.The model of knee osteoarthritis was prepared by injecting sodium iodoacetate into the joint cavity.The successfully modeled rats were divided into model group,sodium hyaluronate group,and electroheating needle group,with 10 rats in each group.After successful modeling,sodium hyaluronate group was injected with 0.1 ml sodium hyaluronate into joint cavity.The electrothermal acupuncture group was treated with electrothermal acupuncture,and the normal group and the model group were given the same volume of normal saline at the same time,once a day,for two weeks.The mechanical pain threshold(PWT)and thermal pain threshold(PWL)in rats were detected.The pathological morphology of cartilage was detected by HE staining.Immunohistochemical staining was used to detect the expressions of glucose regulated protein 78(GRP78)and C/EBP homologous protein(CHOP)in cartilage tissue.Western blot was used to detect the expressions of EGFR,JAK,and STAT proteins,while RT-PCR was used to detect the mRNA levels of GRP78,CHOP,EGFR,JAK,and STAT in cartilage tissue.Results The normal group had normal cartilage tissue structure,orderly distribution of synovial cells,neat arrangement of chondrocytes,and no inflammatory cell infiltration.The model group had synovial hyperplasia and hypertrophy,irregular arrangement of synovial cells and a large number of hyperplasia,disordered arrangement of chondrocytes and increased number of inflammatory cell infiltration.Compared with the model group,the PWT and PWL in cartilage tissues of sodium hyaluronate group and electrothermal acupuncture group increased(P<0.05),the expressions of GRP78 and CHOP in cartilage tissues decreased(P<0.05),the protein expressions of EGFR,JAK1 and STAT1 in cartilage tissue

关 键 词：电热针疗法 骨关节炎 膝 病理性疼痛 内质网应激 EGFR/JAK/STAT信号通路 大鼠 

分 类 号：R73[医药卫生—肿瘤]