慢性乙型肝炎患者血清STAT3和SOX4蛋白水平变化及其与肝纤维化程度的关系探讨  

Analysis of the relationship between the changes of serum STAT3 and SOX4 proteins and the degree of liver fibrosis in patients with chronic hepatitis B

作　　者：邓娟 王秀芳 孙瑞青 DENG Juan;WANG Xiu-fang;SUN Rui-qing(Department of Gastroenterology,First Affiliated Hospital of Air Force Military Medical University,Xi′an,710032,China.)

机构地区：[1]空军军医大学附属第一医院消化内科,陕西西安710032

出　　处：《中西医结合肝病杂志》2025年第3期310-313,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:探究血清信号转导和转录激活因子3(STAT3)和性别决定区Y框4(SOX4)蛋白变化与慢性乙型肝炎(CHB)患者肝纤维化程度的关系。方法:选择2022年10月至2023年10月在空军军医大学附属第一医院消化内科收治的118例CHB患者为CHB组,另选择同期体检的118例健康者为健康组,比较两组对象一般资料;观察CHB患者肝纤维化程度,将肝纤维化分为S1~S4期;采用Western blot法检测两组对象血清STAT3和SOX4蛋白表达水平,Pearson法分析血清STAT3与SOX4水平的相关性,以及血清STAT3和SOX4蛋白表达水平与肝纤维化程度的相关性;受试者工作特征(ROC)曲线分析血清STAT3和SOX4蛋白水平对CHB患者肝纤维化的诊断价值。结果:CHB患者血清STAT3(1.4±0.3)和SOX4(1.2±0.2)水平显著高于健康人(1.0±0.1),(0.9±0.1),差异具有统计学意义(P<0.05);118例CHB患者中,肝纤维化分期为S1期19例,S2期30例,S3期32例,S4期37例,即非显著性患者19例,显著性患者99例。显著性中STAT3(1.4±0.3)、SOX4(1.3±0.3)的表达水平显著高于非显著性(1.01±0.3),(1.0±0.1)(P<0.05);经Pearson相关分析结果显示,血清STAT3与SOX4水平呈正相关(r=0.3,P<0.05),二者表达水平与肝纤维化程度均呈正相关(r=0.4,0.5,P<0.001);ROC曲线显示,STAT3诊断CHB患者肝纤维化程度S2S3S4期、S3S4期、S4期的AUC分别为0.877、0.823、0.937,截断值分别为1.070、1.239、1.411;SOX4诊断CHB患者肝纤维化程度S2S3S4期、S3S4期、S4期的AUC分别0.819、0.950、0.989,截断值分别为1.103、1.210、1.267。结论:CHB患者血清STAT3和SOX4蛋白表达水平随着肝纤维化程度加重而升高,二者表达水平对肝纤维化程度具有一定的诊断价值。Objective:To investigate and analyze the relationship between the changes of serum signal transduction and transcription activator 3(STAT3)and sex-determining region Y-box 4(SOX4)proteins and the degree of liver fibrosis in patients with chronic hepatitis B(CHB).Methods:A total of 118 CHB patients admitted to First Affiliated Hospital of Air Force Military Medical University from October 2022 to October 2023 were selected as the case group,another 118 healthy people in the same period were selected as the control group,the general data of the two groups were compared.The degree of liver fibrosis in the case group was observed and divided into S1-S4 stages.Western blot was used to detect the expression of STAT3 and SOX4 proteins in serum.Pearson method was used to analyze the correlation between serum STAT3 and SOX4 levels,the correlation between serum STAT3 and SOX4 protein expression levels and the degree of liver fibrosis.The ROC curve was used to analyze the diagnostic value of serum STAT3 and SOX4 protein levels for liver fibrosis in patients with CHB.Results:The serum STAT3(1.4±0.3)and SOX4(1.2±0.2)levels in CHB patients were obviously higher than those in the control group(1.0±0.1),(0.9±0.1)(P<0.05).Among 118 patients with CHB,19 cases were in S1 of liver fibrosis,30 cases were in S2,32 cases were in S3,and 37 cases were in S4.There were 19 non significant patients and 99 significant patients.The expression levels of STAT3(1.4±0.3)and SOX4(1.3±0.3)in significance were significantly higher than those in non-significance(1.01±0.3),(1.0±0.1)(P<0.05).Pearson correlation analysis showed that serum STAT3 and SOX4 levels were positively correlated(r=0.3,P<0.05),and there was a positive correlation between the expression levels of both and the degree of liver fibrosis(r=0.4,0.5,P<0.001).The ROC curve showed that the AUC of STAT3 in the diagnosis of S2S3S4,SS3S4,and S4 in patients with CHB was 0.877,0.823,and 0.937 respectively,and the cut-off value was 1.070,1.239,and 1.411 respectively.The AUC of SOX4 in t

关 键 词：信号转导和转录激活因子3 性别决定区Y框4 慢性乙型肝炎 肝纤维化 

分 类 号：R575.1[医药卫生—消化系统]