基于p38 MAPK/IL-17信号通路探讨茵陈有效成分Scoparone对肝衰竭炎症微环境的作用机制  

Exploring the mechanism of Scoparone,the active ingredient of Artemisia arborescens,on the inflammatory microenvironment of liver failure based on the p38 MAPK/IL-17 signaling pathway

作　　者：刘英[1] 俞姝丹 杨玉雯 王光耀 夏猛[1] 吕建林[2] LIU Ying;YU Shu-dan;YANG Yu-wen;LV Jian-lin(The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning Guangxi,530023,China)

机构地区：[1]广西中医药大学 [2]广西中医药大学第一附属医院,广西南宁530023

出　　处：《中西医结合肝病杂志》2025年第3期329-334,共6页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：中国博士后科学基金面上项目(No.2023MD734157);广西自然科学基金资助项目(No.2020GXNSFAA238020,2022GXNSFAA035446);广西重点研发项目(No.桂科AB23026137);广西研究生教育创新计划资助项目(No.YCSY2023028)。

摘　　要：目的:通过观察茵陈有效成分6,7-二甲氧基香豆素(Scoparone/Sco)干预肝衰竭大鼠模型前后相关指标变化情况,探讨Sco对肝衰竭大鼠肝脏炎症微环境的作用机制。方法:随机将60只SD大鼠分为3组:Con组、Model组、Sco组各20只。Sco组给予5 ml/kg DMSO溶液和60 mg/kg Scoparone处理,Con组和Model组大鼠给予等量生理盐水处理,均连续干预7天。Model组与Sco组采用D-氨基半乳糖联合脂多糖,腹腔注射一次成模。采集各组大鼠腹主动脉血及肝组织,肝功能(ALT、AST、TBil)及炎症因子(IL-6、IL-17、IL-21)进行检测,Western blotting及荧光定量qPCR检测肝组织p38 MAPK、IL-17、AP-1蛋白与基因的表达情况。结果:与Con组相比,Model组ALT、AST、TBil、IL-6、IL-17、IL-21均显著升高(P<0.05);与Model组相比,Sco组ALT、AST、TBil、IL-6、IL-17、IL-21均显著下降(P<0.05)。与Con组相比,Model组肝组织p38 MAPK、IL-17、AP-1蛋白与mRNA表达均显著升高(P<0.05);与Model组相比,Sco组肝组织p38 MAPK、IL-17、AP-1蛋白与mRNA表达均显著下降(P<0.05)。结论:Sco可能通过调控p38 MAPK/IL-17信号通路,下调p38 MAPK、IL-17、AP-1表达,进而抑制肝衰竭炎症反应。Objective:To investigate the mechanism of Sco on the liver inflammatory microenvironment of rats with liver failure by observing the changes of relevant indexes of Yinchen's active ingredient 6,7-dimethoxycoumarin(Scoparone/Sco)before and after intervention in a rat model of liver failure.Methods:Sixty SD rats were randomly divided into three groups:blank group,model group,and Sco group,with twenty 20 rats in each group.The Sco group is treated with 5ml/kg DMSO solution and 60mg/kg Scoparone,and the rats in the blank group and model group are treated with the same amount of normal saline,both of which are continuously intervened for seven days.The model group and the Sco group were modeled with D-aminogalactose combined with lipopolysaccharide,and intraperitoneal injection was used once.The abdominal aortic blood and liver tissues of rats in each group were collected for the detection of liver function ALT,AST,TBil,inflammatory cytokines IL-6,IL-17 and IL-21,and the expressions of p38 MAPK,IL-17 and AP-1 proteins and genes were detected by Western blotting and fluorescence quantitative qPCR.Results:Compared with the blank group,ALT,AST,TBil,IL-6,IL-17,and IL-21 were significantly increased in the model group(P<0.05),and compared with the model group,ALT,AST,TBil,IL-6,IL-17,and IL-21 in the Sco group were significantly decreased(P<0.05).Compared with the blank group,the expressions of p38 MAPK,IL-17,and AP-1 protein and mRNA in the model group were significantly increased(P<0.05),and compared with the model group,the expressions of p38 MAPK,IL-17 and AP-1 protein and mRNA in the Sco group were significantly decreased(P<0.05).Conclusion:Sco may regulate the p38 MAPK/IL-17 signaling pathway,down-regulate the expression of p38 MAPK,IL-17,and AP-1,and then inhibit the inflammatory response of liver failure.

关 键 词：6 7-二甲氧基香豆素 p38 MAPK/IL-17通路 肝衰竭 炎症微环境 

分 类 号：R575.3[医药卫生—消化系统]