光声显微成像技术在眼科的应用进展  

Advances in the application of photoacoustic microscopy imaging in ophthalmology

作　　者：刘楚 张正威 Liu Chu;Zhang Zhengwei(Jiangnan University Medical Center/Wuxi No.2 People's Hospital,Wuxi 214002,Jiangsu Province,China)

机构地区：[1]江南大学附属中心医院、无锡市第二人民医院,江苏省无锡市214002

出　　处：《国际眼科杂志》2025年第4期606-610,共5页International Eye Science

基　　金：江苏省科技厅社会发展面上研发项目(No.BE2022699);江苏省卫健委老年医学面上项目(No.LKM2022043);无锡市卫生健康委中青年拔尖人才资助计划(No.BJ2023037)。

摘　　要：光声显微成像(PAM)是一种融合光学与声学的新兴的非侵入性活体组织成像技术,能够高分辨率和高对比度地呈现眼部各组织结构和功能信息,可应用于角膜新生血管、黄斑变性、糖尿病视网膜病变等多种眼部疾病研究。PAM的多波长光源成像特性使其在早期病变筛查和动态生理监测中具有重要应用价值。在干细胞追踪中,PAM利用造影剂标记实现移植细胞的动态监测。此外,与光相干断层扫描(OCT)等多模态成像技术联合使用,能够提升眼部病变的检测精度和诊断能力。然而,PAM在成像速度和造影剂安全性等方面仍需优化。文章综述了PAM的基本原理与发展历程,探讨了其在部分眼部疾病中的应用,并分析了从动物实验到临床应用的挑战及优化方向。PAM有望在眼科诊断与治疗中发挥更重要的作用。Photoacoustic microscopy(PAM)is an emerging,non-invasive,in vivo imaging modality that merges optical and acoustic principles.It offers high-resolution and high-contrast visualization of various ocular tissue structures and functional information,making it suitable for studying a wide range of ophthalmic diseases such as corneal neovascularization,macular degeneration,and diabetic retinopathy.The multi-wavelength illumination capability of PAM makes it particularly valuable for early disease screening and dynamic physiological monitoring.In stem cell tracking,PAM enables the dynamic monitoring of transplanted cells through contrast agent labeling.Moreover,when combined with multimodal imaging techniques like optical coherence tomography(OCT),PAM can enhance the detection accuracy and diagnostic capacity for ocular diseases.However,PAM still requires optimization in terms of imaging speed and contrast agent safety.This review summarizes the fundamental principles and development of PAM,explores its applications in specific ophthalmic diseases,and analyzes the challenges and optimization directions from animal experiments to clinical applications.PAM holds great promise for playing a more significant role in ophthalmic diagnosis and treatment.

关 键 词：光声显微成像 眼部疾病 诊断 评估 应用 

分 类 号：R73[医药卫生—肿瘤]