糖尿病视网膜病变患者外周血中炎症细胞因子和CD8^(+)T细胞亚群的表达水平变化  

Changes in the expression levels of inflammatory cytokines and CD8^(+)T cell subsets in the peripheral blood of patients with diabetic retinopathy

作　　者：蒋文君 赵柏林 马善波 马为梅 王至允 于敬妮 李娅 Jiang Wenjun;Zhao Bolin;Ma Shanbo;Ma Weimei;Wang Zhiyun;Yu Jingni;Li Ya(Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi Province,China;Department of Clinical Laboratory,Xi'an People's Hospital(Xi'an Fourth Hospital),Xi'an 710004,Shaanxi Province,China;Department of Biochemistry and Molecular Biology,Xi'an Jiaotong University Health Science Center,Xi'an 710061,Shaanxi Province,China;Department of Pharmacy,Xijing Hospital,Air Force Medical University,Xi'an 710032,Shaanxi Province,China;Department of Ophthalmology,Xi'an People's Hospital(Xi'an Fourth Hospital),Xi'an 710004,Shaanxi Province,China)

机构地区：[1]陕西中医药大学,陕西省咸阳市712046 [2]中国陕西省西安市人民医院(西安市第四医院)临床检验中心,710004 [3]西安交通大学医学部生物化学与分子生物学系,陕西省西安市710061 [4]空军军医大学西京医院药学部,陕西省西安市710032 [5]中国陕西省西安市人民医院(西安市第四医院)眼科,710004

出　　处：《国际眼科杂志》2025年第4期638-643,共6页International Eye Science

摘　　要：目的:探讨糖尿病视网膜病变(DR)患者外周血中炎症细胞因子和CD8^(+)T细胞亚群的表达水平变化。方法:回顾性研究。选取2022-04/07西安市人民医院(西安市第四医院)收治的2型糖尿病患者40例,根据患者视网膜是否发生病变分为单纯2型糖尿病(DM)组20例和糖尿病视网膜病变(DR)组20例,另选取同一时间体检正常20例作为对照组。采用ELISA法检测所有参与者外周血中IL-6、IL-8、IL-10细胞因子的表达水平;流式细胞术分析CD8^(+)T细胞中PD-1、TIM-3、CD28、CD57分子表达水平。结果:ELISA法检测出DR患者外周血IL-6、IL-8、IL-10炎症细胞因子表达较DM组和对照组均显著升高(均P<0.001);流式细胞术分析显示,DR患者外周血CD8^(+)T细胞中PD-1、TIM-3、CD57表达较DM组和对照组均升高(均P<0.001),CD28表达均降低(均P<0.001)。结论:DR患者体内可能由于IL-6、IL-8、IL-10促炎细胞因子的表达增多导致CD8^(+)T细胞出现耗竭与衰老。AIM:To investigate alterations in the expression levels of inflammatory cytokines and subsets of CD8^(+)T cells in the peripheral blood of patients with diabetic retinopathy(DR).METHODS:Retrospective study.A total of 40 patients with type 2 diabetes admitted to Xi’an People’s Hospital(Xi’an Fourth Hospital)from April to July 2022 were recruited for this study and categorized into two groups:20 cases in the simple type 2 diabetes mellitus(DM)group,and 20 cases in the DR group.Additionally,20 healthy individuals undergoing routine physical examinations served as the control group.The expression levels of cytokines,including interleukin(IL)-6,IL-8,and IL-10 in peripheral blood were quantified using ELISA.Flow cytometry was employed to analyze the expression of programmed cell death-1(PD-1),T cell immunoglobulin domain and mucin domain protein-3(TIM-3),CD28,and CD57 on CD8^(+)T cells.RESULTS:The peripheral blood expression of IL-6,IL-8,and IL-10 inflammatory cytokines were significantly elevated in DR patients as detected by ELISA(all P<0.001);flow cytometry analysis showed that the expression of PD-1,TIM-3,and CD57 were elevated in peripheral blood CD8^(+)T cells of DR patients(all P<0.001),and the expression of CD28 was decreased(all P<0.001).CONCLUSION:In DR patients,CD8^(+)T cells may undergo depletion and senescence as a result of elevated pro-inflammatory cytokines,including IL-6,IL-8,and IL-10.

关 键 词：糖尿病视网膜病变 流式细胞术 T细胞耗竭 

分 类 号：R73[医药卫生—肿瘤]