LINC00365靶向调控miR-519d影响甲状腺癌细胞的增殖、凋亡、迁移与侵袭  

Effect of LINC00365 on Proliferation,Apoptosis,Migration,and Invasion of Thyroid Cancer Cells by Targeting miR-519d

作　　者：吾甫尔·依马尔[1] 王护国[1] 达尔汗·赛力克别克 邓超 马依热·努尔买卖提 Wufuer Yimaer;WANG Huguo;Daerhan Sailikebieke;DENG Chao;MaYire Nuermaimaiti(Department of Vascular and Thyroid Surgery,First Affiliated Hospital of Xinjiang Medical University,Urumqi,830054,China;Animal Experiment Center,Xinjiang Medical University,Urumqi,830054,China)

机构地区：[1]新疆医科大学第一附属医院血管甲状腺外科,乌鲁木齐市830054 [2]新疆医科大学动物实验中心,乌鲁木齐市830054

出　　处：《医学分子生物学杂志》2025年第2期117-123,138,共8页Journal of Medical Molecular Biology

基　　金：新疆维吾尔自治区自然科学基金(No.2021D01C325)。

摘　　要：目的探究长非编码RNALINC00365对甲状腺癌细胞增殖、凋亡、迁移与侵袭的影响及机制。方法qRT-PCR检测甲状腺癌组织和癌旁组织以及甲状腺癌细胞株中LINC00365的相对表达量。甲状腺癌细胞BHP-10-3分为si-NC组、si-LINC00365组、si-LINC00365+NC inhibitor组、si-LINC00365+miR-519d inhibitor组、空白组,双荧光素酶报告基因实验验证LINC00365与miR-519d的靶向关系。CCK8检测细胞增殖能力,细胞划痕检测细胞迁移能力,Transwell小室检测细胞侵袭能力,流式细胞术检测细胞凋亡率。结果LINC00365在甲状腺癌组织及甲状腺癌细胞中相对表达量显著上调。miR-519d为LINC00365靶基因。相比于空白组,si-LINC00365组和si-LINC00365+NC inhibitor组BHP-10-3增殖、迁移和侵袭能力显著降低(P<0.05),细胞凋亡率显著增加(P<0.01)。相比于si-LINC00365组,si-LINC00365+miR-519d inhibitor组BHP-10-3增殖、迁移和侵袭能力显著增加(P<0.05),细胞凋亡率显著降低(P<0.01)。结论LINC00365靶向调控miR-519d影响甲状腺癌细胞的增殖、迁移、侵袭和凋亡。Objective To investigate the effects and mechanisms of LINC00365 on proliferation,apoptosis,migration and invasion of thyroid cancer cells.Methods qRT-PCR was used to detect the relative expression level of LINC00365 in thyroid cancer tissues and thyroid cancer cell lines.Thyroid cancer cells BHP-10-3 were divided into 5 groups:siNC group,si-LINC00365 group,si-LINC00365+NC inhibitor group,si-LINC00365+miR-519d inhibitor group,and blank group.Dual luciferase reporter gene experiment was used to detect the targeting relationship between LINC00365 and miR-519d.Cell proliferation ability was detected by CCK8,cell migration ability was detected by wound healing assay,cell invasion ability was detected by transwell assay,apoptosis rate was detected by flow cytometry.Results The relative expression level of LINC00365 were significantly upregulated in thyroid cancer tissues and thyroid cancer cells.miR-519d is the target gene of LINC00365.the proliferation,migration,and invasion abilities of BHP-10-3 in the si-LINC00365 group and the si-LINC00365+NC inhibitor group were significantly reduced(P<0.05),and the cell apoptosis rate was significantly increased(P<0.01)when compared with those in the blank group.The proliferation,migration,and invasion abilities of BHP-10-3 in the si-LINC00365+miR-519d inhibitor group were significantly increased(P<0.05),and the cell apoptosis rate was significantly reduced(P<0.01)when compared with those in the si-LINC00365 group.Conclusion LINC00365 can target miR-519d to affect the proliferation,migration,invasion and apoptosis of thyroid cancer cells.

关 键 词：LINC00365 甲状腺癌 增殖 凋亡 迁移 侵袭 

分 类 号：R394.3[医药卫生—医学遗传学]