胃癌根治术联合洛铂对胃癌患者T细胞、ONECUT2及YAP水平的影响  

Effects of radical gastrectomy combined with loplatin on T cells,ONECUT2 and YAP levels in patients with gastric cancer

作　　者：徐毅[1] 唐岩 韩栓柱 李冬杨 张欣萍[1] XU Yi;TANG Yan;HAN Shuanzhu;LI Dongyang;ZHANG Xinping(Department of Gastrointestinal Surgery,Nanyang First People's Hospital,Nanyang,Henan,China,473000)

机构地区：[1]南阳市第一人民医院胃肠外科,河南南阳473000

出　　处：《分子诊断与治疗杂志》2025年第2期366-369,373,共5页Journal of Molecular Diagnostics and Therapy

基　　金：江苏省放射医学与防护重点实验室开放课题(KJS2242)。

摘　　要：目的探究胃癌根治术联合洛铂对胃癌患者T细胞、ONECUT2(OC-2)及YAP水平的影响。方法选取2021年3月至2022年2月于南阳市第一人民医院行治疗的胃癌患者82例,按照不同治疗方法分为对照组(胃癌根治术^(+)卡培他滨)41例、联合组(胃癌根治术^(+)卡培他滨^(+)洛铂化疗)41例。采用脱氧核苷酸末端转移酶(TUNEL)检测细胞凋亡率;采用流式细胞仪检测CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)T;采用免疫印迹检测胃癌组织中p-YAP、YAP、OC-2水平;参照实体瘤疗效评价标准RECIST评估临床疗效;随访2年,记录患者2年生存率。结果对照组胃癌组织中细胞凋亡积分光密度值低于联合组,差异有统计学意义(P<0.05);化疗前,对照组及联合组CD3^(+)、CD4^(+)、CD8^(+)比较差异无统计学意义(P>0.05);治疗后1 d,与对照组相比,联合组CD3^(+)、CD4^(+)降低,CD8^(+)升高,差异具有统计学意义(P<0.05);治疗后2周,对照组及联合组CD3^(+)、CD4^(+)、CD8^(+)比较差异无统计学意义(P>0.05);对照组p-YAP、YAP、OC-2蛋白表达水平高于联合组,差异有统计学意义(P<0.05);对照组治疗有效率低于联合组,差异有统计学意义(P<0.05);两组患者均进行2年随访,中途无脱落,对照组死亡21例,生存20例,生存率为48.78%;联合组死亡12例,生存29例,生存率为70.73%,联合组2年生存率高于对照组,差异有统计学意义(P<0.05)。结论胃癌根治术联合洛铂治疗可调控胃癌患者YAP表达,提高疗效及生存率,减少免疫功能损伤。Objective To investigate the effects of radical gastrectomy combined with loplatin on T cells,ONECUT2(OC-2)and YAP levels in patients with gastric cancer.Methods A total of 82 patients with gastric cancer who were treated at Nanyang First People's Hospital from March 2021 to February 2022 were selected and divided into a control group(radical gastrectomy^(+)capecitabine)with 41 cases and a combination group(radical gastrectomy^(+)capecitabine^(+)loplatin chemotherapy)with 41 cases according to different treatment methods.The apoptosis rate was detected by Term inal2deoxynucleo t idyl t ran sferase(TUNEL).CD3^(+),CD4^(+),CD8^(+),CD4^(+)/CD8^(+)T were detected by flow cytometry.The levels of p-YAP,YAP and OC-2 in gastric cancer tissues were detected by Western blot.The clinical efficacy was evaluated according to RECIST,the standard of solid tumor efficacy evaluation.The patients were followed up for 2 years and the 2-year survival rate was recorded.Results The integral optical density of apoptosis in the control group was lower than that in the combined group,and the difference was statistically significant(P<0.05).Before chemotherapy,there was no significant difference in CD3^(+),CD4^(+)and CD8^(+)between the control group and the combination group(P>0.05).One day after treatment,CD3^(+)and CD4^(+)were decreased and CD8^(+)were increased in the combination group compared with the control group,the difference was statistically significant(P<0.05).Two weeks after treatment,there was no significant difference in CD3^(+),CD4^(+)and CD8^(+)between the control group and the com bination group(P>0.05).The protein expression levels of P-YAP,YAP,and OC-2 in the control group were higher than those in the combination group,and the difference was statistically significant(P<0.05).The treatment effective rate in the control group was lower than that in the combination group,the difference was statistically significant(P<0.05).The patients in both groups were followed up for 2 years,and there was no shedding during the course

关 键 词：胃癌根治术 洛铂 胃癌患者 T细胞 YAP 

分 类 号：R735.2[医药卫生—肿瘤]