厚皮甜瓜心部果肉可溶性固形物含量遗传规律分析及QTL定位  

QTL mapping and genetic analysis of soluble solids content in the center flesh of muskmelon

作　　者：范蓉 张永兵[1] 李寐华[1] 张学军[1] 伊鸿平[1] 刘钊 杨永[1] FAN Rong;ZHANG Yongbing;LI Meihua;ZHANG Xuejun;YI Hongping;LIU Zhao;YANG Yong(Center of Hami Melon,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Shouguang Sanmu Seed Co.,Ltd,Shouguang Shandong 262700,China)

机构地区：[1]新疆农业科学院哈密瓜研究中心,乌鲁木齐830091 [2]山东省寿光市三木种苗有限公司,山东寿光262700

出　　处：《新疆农业科学》2025年第1期182-192,共11页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重点研发项目(2022B02002-3);国家自然科学基金项目(31860567);财政部和农业农村部"国家现代农业产业技术体系资助"(CARS-25-G2);新疆农业科学院青年科技骨干创新能力培养项目(xinkq-2022011);新疆维吾尔自治区重大科技专项(2022A03004-5);海南省院士创新平台科研专项(吴明珠院士团队创新中心);新疆特色果蔬基因组研究与遗传改良重点实验室。

摘　　要：【目的】研究厚皮甜瓜果实心部可溶性固形物的遗传规律和QTL定位,挖掘厚皮甜瓜果实心部可溶性固形物相关的候选基因。【方法】以高糖材料P 1和低糖材料P 2为双亲构建六世代分离群体;采用主基因+多基因混合遗传模型研究厚皮甜瓜心部果肉可溶性固形物含量的遗传规律,并基于F 2群体,选取果实可溶性固形物含量极端的单株构建混池,利用BSA方法对甜瓜可溶性固形物含量进行定位。【结果】甜瓜可溶性固形物符合E-1(MX2-ADI-AD)遗传模型,2对主基因以上位性效应为主,其次为显性效应、加性效应。2个QTL分别在第5号染色体827066 bp-109953 bp和第8号11316600 bp-11729324 bp,区间大小分别为0.27 Mb和0.41 Mb,2个区间内共包含50个候选基因。筛选获得了6个与甜瓜可溶性固形物含量相关的候选基因,分别是MELO3C014619、MELO3C014617、MELO3C014596、MELO3C014594、MELO3C019077、MELO3C019089。【结论】厚皮甜瓜心部可溶性固形物符合E-1(MX2-ADI-AD)遗传模型,通过BSA方法在5号和8号染色体定位到2个甜瓜可溶性固形物相关QTL,筛选出6个与甜瓜可溶性固形物含量相关的候选基因。【Objective】This study aims to analyze the content of soluble solids in the center flesh of muskmelon by Genetic analysis and QTL mapping in the hope of finding out candidate genes associated with soluble solids in the heart of C.achyum fruits.【Methods】Six generations population(P 1,P 2,F 1,B 1,B 2,and F 2)were created using the high-sugar material P 1 and the low-sugar material P 2 as parents.A mixed major gene plus polygene inheritance model was used to analyze soluble solids content in the center flesh of six generations population.Based on the F 2 population,individual plants with higher and lower fruit soluble solids content were selected to construct two pools to locate the soluble solids content of muskmelons by using the BSA method.【Results】Muskmelon soluble solids conformed to the E-1(MX2-ADI-AD)genetic model,with epistatic effects dominating the 2 main gene pairs,followed by dominant and additive effects.Two QTLs were located in chromosome 5(827066 bp-109953bp)and chromosome 8(11316600 bp-11729324 bp),with interval sizes of 0.27Mb and 0.41 Mb,respectively,and contained a total of 50 candidate genes.Six candidate genes related to soluble solids content of melon were screened and obtained(MELO3C014619,MELO3C014617,MELO3C014596,MELO3C014594,MELO3C019077 and MELO3C019089).【Conclusion】Muskmelon soluble solids conforms to the E-1(MX2-ADI-AD)genetic model,two QTLs related to soluble solid content of muskmelon are mapped on chromosomes 5 and 8 by BSA method,and six candidate genes related to soluble solid content of muskmelon screened out.

关 键 词：厚皮甜瓜 可溶性固形物 主基因+多基因 BSA 

分 类 号：S652[农业科学—果树学]