改性糠醛渣介导根际微生物缓解制种玉米连作障碍研究  

Modified furfural residue mediated rhizosphere microbial alleviated obstacles of continuous corn cropping

作　　者：赵芸晨 甫文疆 宋学林 ZHAO Yunchen;FU Wenjiang;SONG Xuelin(School of Agricultural and Ecological Engineering,Hexi University,Zhangye 734000,China;College of Resources and Environment,Northwest A&F University,Yangling 712100,China;Zhangye Seed Administration Bureau of Gansu Province,Zhangye 734000,China)

机构地区：[1]河西学院农业与生态工程学院,甘肃张掖734000 [2]西北农林科技大学资源与环境学院,陕西杨凌712100 [3]甘肃省张掖市种子管理局,甘肃张掖734000

出　　处：《南京农业大学学报》2025年第2期380-391,共12页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(41867010);甘肃省重点研发项目(22YF7NG128);甘肃省教育厅产业支撑项目(2023CYZC-65)。

摘　　要：[目的]本文以不同连作年限地块(5~30 a)为研究对象,旨在探究施用改性糠醛渣对制种玉米连作障碍的影响。[方法]通过大田试验及高通量测序,测定不同连作年限、不同生育期制种玉米连作根际微生物群落结构及功能变化特征,分析连作障碍机制及缓解诱因。[结果]制种玉米连作显著降低根际微生物群落多样性,微生物群落结构定向改变;COG库预测表征遗传信息处理、代谢过程、细胞过程、信号转导机制及防御机制的相关二级通路显著下降,微生物功能定向改变。施用改性糠醛渣提高连作制种大田根际微生物群落多样性与网络复杂度,多样性指数显著增加,群落共有OTU及特有OTU显著增加,网络边及平均度增加,根际Lysobacter、Sphingomonas、Nitrospira、Bryobacter、Flavisolibacter、Adhaeribacter、Pseudarthrobacter、Ohtaekwangia、Pseudomonas、Chaetomium及Trichoderma等属相对丰度上升,而病原性Penicillium、Aspergillus、Verticillium、Alternaria及Microdochium等属相对丰度下降。[结论]施用改性糠醛渣介导制种玉米连作体系,促进有益微生物增加,病原微生物减少,根际微生物群落重新组装,根际微生物群落多样性增加,网络复杂度增加,根际细菌功能显著提高,极大消除连作带来的负面效应,从而缓解制种玉米连作导致的连作障碍。[Objectives]In order to explore the effects of exogenous organic carbon application on obstacle of continuous corn cropping,different continuous cropping field(5-30 a)with modified furfural residue application were studied.[Methods]Through field experiment and high-throughput sequencing,the structure and functionality of rhizosphere microbiome were determined,and the mechanism of continuous cropping obstacles and mitigation factors were analyzed.[Results]The rhizosphere microbial community diversity significantly decreased and the microbial community structure orienteering changed.The secondary pathways representing functional genetic information processing,metabolic processes,cellular processes,signal transduction mechanisms and defense mechanisms decreased significantly,and microbial functions changed with continuous cropping.While application of furfural residue improved rhizosphere microbial community diversity and network complexity in continuous cropping field.The diversity index,common OTU and unique OTU increased significantly.Network edge and average increased either.The relative abundance of Lysobacter,Sphingomonas,Nitrospira,Bryobacter,Flavisolibacter,Adhaeribacter,Pseudarthrobacter,Ohtaekwangia,Pseudomonas,Chaetomium and Trichoderma increased.While the relative abundance of the pathogenic Penicillium,Aspergillus,Verticillium,Alternaria and Microdochium decreased.[Conclusions]Modified furfural residue application mediated continuous corn cropping,increased beneficial microorganisms,decreased pathogenic microorganisms,rhizosphere microbial community reassembled,rhizosphere microbial diversity and network complexity increased,and function of rhizosphere bacteria significantly improved,which greatly eliminated the negative effects of continuous cropping of corn seed production,thus,the obstacles can be alleviated.

关 键 词：制种玉米 连作 改性糠醛渣 根际微生物 微生物多样性 

分 类 号：S143.1[农业科学—肥料学] S154.3[农业科学—农业基础科学]