末水条斑紫菜对健康C57BL/6J小鼠肠道微生物的影响  

Effect of last stage Porphyra yezoensis on intestinal microbiota of healthy C57BL/6J mice

作　　者：程诗瑶 吴越[1] 陈志刚[1] CHENG Shiyao;WU Yue;CHEN Zhigang(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学食品科学技术学院,江苏南京210095

出　　处：《南京农业大学学报》2025年第2期454-463,共10页Journal of Nanjing Agricultural University

基　　金：江苏现代农业(紫菜)产业技术体系质量安全与加工创新团队(JATS[2023]434)。

摘　　要：[目的]本文旨在探究末水条斑紫菜对健康C57BL/6J小鼠肠道微生物及其代谢产物的影响。[方法]对小鼠分别灌胃生理盐水(对照组)、1.15 g·kg^(-1)(低剂量组)和2.30 g·kg^(-1)(高剂量组)剂量的末水条斑紫菜,28 d后取小鼠标本进行病理切片观察、短链脂肪酸含量测定以及肠道菌群16S rDNA高通量测序。[结果]灌胃末水条斑紫菜组小鼠生长性能没有显著性变化。与对照组结肠长度53.50 mm相比,低剂量组小鼠结肠显著增长至65.60 mm;结肠组织病理切片显示,末水条斑紫菜可以增加杯状细胞的数量。通过对结肠内容物中短链脂肪酸含量测定,发现乙酸、丙酸、正戊酸与异戊酸含量均没有显著变化,低剂量组小鼠结肠内容物中正丁酸含量显著增加,高剂量组小鼠结肠内容物中正丁酸与异丁酸含量均显著增加。经16S rDNA高通量测序小鼠结肠内容物发现,灌胃低剂量与高剂量末水条斑紫菜均可降低微生物物种多样性,增加厚壁菌门的相对丰度,降低拟杆菌门的相对丰度。但肠道中杜氏杆菌属(Dobusiella)相对占比显著增加,Norank_f_norank_o_Clostridia_UCG-014、螺杆菌(Helicobacter)等致病菌相对占比减少。[结论]末水条斑紫菜能够促进结肠组织长度变化,调节健康C57BL/6J小鼠肠道菌群,促进有益菌生长以及丁酸的产生,最佳摄入量为1.15 g·kg^(-1)。[Objectives]The aim of this study was to investigate the effects of last stage Porphyra haitanensis on the intestinal microbiota and their metabolic products in healthy C57BL/6J mice.[Methods]The mice were gavaged with saline(control group),1.15 g•kg^(-1)(low-dose group),and 2.30 g•kg^(-1)(high-dose group)doses of last stage P.yezoensis.After 28 days,mouse samples were collected for pathological section observation,short-chain fatty acid content determination,and intestinal microbiota 16S rDNA high-throughput sequencing.[Results]The results indicated no significant changes in the growth performance of mice gavaged with last stage P.haitanensis.Compared to the control group with a colon length of 53.50 mm,the colon length in the low-dose group significantly increased to 65.60 mm.Pathological sections of the colon tissue revealed that last stage P.haitanensis increased the number of goblet cells.The results of short-chain fatty acids in the colonic contents showed no significant changes in the levels of acetic acid,propionic acid,n-valeric acid,and isovaleric acid,while the level of butyric acid of low-dose group significantly increased,and the levels of butyric acid and isobutyric acid of high-dose group significantly increased.The result of 16S rDNA in the colonic contents found that both low-dose and high-dose administration of last stage P.yezoensis reduced the diversity of intestinal microbial diversity,increased the relative abundance of Firmicutes and decreased that of Bacteroidetes.However,the relative proportion of the genus Dubosiella significantly increased.Pathogens such as Norank_f_norank_o_Clostridia_UCG-014 and Helicobacter showed a decreased relative abundance.[Conclusions]Last stage P.haitanensis could regulate the intestinal microbiota of healthy C57BL/6J mice,promoting the growth of beneficial bacteria and the production of butyric acid.The optimal intake was 1.15 g•kg^(-1).

关 键 词：末水条斑紫菜 健康C57BL/6J小鼠 肠道菌群 短链脂肪酸 

分 类 号：TS201.4[轻工技术与工程—食品科学]