Survivin调控小细胞肺癌细胞生物学行为及CD8^(+)T细胞肿瘤杀伤功能研究  

Research of Survivin regulates the biological behavior of small cell lung cancer cells and the tumor killing function of CD8^(+)T cells

作　　者：卡迪丽娅·阿不都卫力 惠菁 阿孜古丽·吐尔逊买买提 麦热哈巴·哈力克 KADILIA·Abuduweili;HUI Jing;AZIGULI·Tuersunmaimaiti;MAIREHABA·Halike(Department of Respiratory and Critical Care Medical Center,Xinjiang Uygur Autonomous Region People′s Hospital,Urumqi,Xinjiang 830000,China)

机构地区：[1]新疆维吾尔自治区人民医院呼吸与危重症医学中心,新疆乌鲁木齐830000

出　　处：《国际检验医学杂志》2025年第6期675-680,共6页International Journal of Laboratory Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C140)。

摘　　要：目的探讨生存素(Survivin)对小细胞肺癌细胞恶性生物学行为的影响。方法将NCI-H82细胞分为Vector组、Survivin组,NCI-H209细胞分为si-NC组、si-Survivin组,采用CCK-8和流式细胞术检测细胞增殖和凋亡情况,实时荧光定量PCR及蛋白质印迹法检测程序性死亡受体配体1(PD-L1)表达。CD8^(+)T细胞与各组NCI-H82和NCI-H209细胞共孵育后,酶联免疫吸附试验检测上清液中干扰素γ(IFN-γ)、白细胞介素-2(IL-2)水平,细胞毒性试验检测肿瘤细胞裂解率。结果与Vector组比较,Survivin组NCI-H82细胞PD-L1表达和细胞增殖能力均升高(P<0.05),细胞凋亡率降低(P<0.001)。与si-NC组比较,si-Survivin组NCI-H209细胞PD-L1表达和细胞增殖能力均降低(P<0.001),细胞凋亡率升高(P<0.001)。与Vector+CD8^(+)T组比较,Survivin+CD8^(+)T组细胞上清液中IFN-γ、IL-2水平及细胞裂解率均降低(P<0.001)。与si-NC+CD8^(+)T组比较,si-Survivin+CD8^(+)T组细胞上清液中IFN-γ、IL-2水平及细胞裂解率均升高(P<0.001)。结论Survivin调控小细胞肺癌的增殖、凋亡,并且上调PD-L1表达抑制CD8^(+)T细胞杀伤功能。Objective To investigate the effect of Survivin on the malignant biological behavior of small cell lung cancer cells.Methods NCI-H82 cells were divided into Vector group and Survivin group,and NCI-H209 cells were divided into si-NC group and si-Survivin group.Cell proliferation and apoptosis were detected by CCK-8 and flow cytometry.Real-time fluorescent quantitative PCR and Western blot were used to detect the expression of programmed death ligand 1(PD-L1).After co-incubation of CD8^(+)T cells with NCI-H82 and NCI-H209 cells,the levels of interferon-γ(IFN-γ)and interleukin-2(IL-2)in the supernatant were detected by enzyme-linked immunosorbent assay,and the lysis rate of tumor cells was detected by cytotoxicity assay.Results Compared with the Vector group,the expression of PD-L1 and the proliferation of NCI-H82 cells in the Survivin group were significantly increased(P<0.05),and the apoptosis rate was decreased(P<0.001).Compared with the si-NC group,the expression of PD-L1 and the proliferation of NCI-H209 cells in the si-Survivin group were significantly decreased(P<0.001),and the apoptosis rate of NCI-H209 cells was increased(P<0.001).Compared with the Vector+CD8^(+)T group,the levels of IFN-γand IL-2 in the supernatant of Survivin+CD8^(+)T cells and the cell lysis rate were significantly decreased(P<0.001).Compared with si-NC+CD8^(+)T group,the levels of IFN-γand IL-2 in the supernatant and the lysis rate of si-Survivin+CD8^(+)T group were significantly increased(P<0.001).Conclusion Survivin can regulate the proliferation and apoptosis of small cell lung cancer,and up-regulate the expression of PD-L1 to inhibit the killing function of CD8^(+)T cells.

关 键 词：生存素 程序性死亡受体配体1 CD8^(+)T细胞 小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]