Computational insights into CRISP3 downregulation in cervical cancer and its cervical lineages pattern  

作　　者：Ricardo Cesar Cintra Andres Galindo Céspedes Mércia Patricia Ferreira Conceicao Maiza Vitoria Aguiar Silva OliveiraAlessandro Buron Deisiane Rodrigues das Neves Fabio Alves Moraes Olinda Maria Gamarra Daniel Rodrigues de Bastos 

机构地区：[1]Department of Oncology,Universidade de Sao Paulo,Sao Paulo 71.961-540,Brazil [2]Clinical Pathology Service,Almanzor Aguinaga Asenjo National Hospital,Chiclayo 14001,Peru [3]Universidad Privada delEste,Alto Parana 7000,Paraguay

出　　处：《Precision Clinical Medicine》2024年第3期246-256,共11页精准临床医学(英文)

摘　　要：Background:Cysteine-rich secretory protein 3(CRISP3)emerges as a potential biomarker in the study of many cancers,including cervical cancer(CC).This study aimed to analyze the expression pattern of CRISP3 in CC patients and CC cell lineages,following treat-ment with the epigenetic drugs:trichostatin A(TSA)and 5-aza-2'-deoxycytidine(5-aza).Methods:The differentially expressed genes identified in GSE63514 were used to construct a protein-protein interaction network.CRISP3 was selected for subsequent analyses.We utilized data from the TCGA and GENT2 projects to evaluate the expression profile and clinical behavior of CRiSP3.Addition-ally,we conducted cell culture experiments to analyze the expression profile of CRISP3 in cells.Results:Low levels of CRISP3 were observed in squamous cell carcinoma(SCC)and human papilomavirus(HPV)16+,along with being associated with worse overall survival(OS).MIR-1229-3p was analyzed,and its high expression was associated with worse prognostic outcomes.In CC-derived cell lines,we observed low levels of CRISP3 in SiHa,followed by SW756,C33A,HeLa,and higher levels in CaSki.All cells were treated with TSA,5-aza,or both.In all cell lines,treatment with TSA resulted in increased transcription of CRISP3.Conclusion:We identified a significant downregulation of CRISP3 in CC,particularly in cases with HPV16 infection and SCC,which was associated with poorer OS.Preliminary findings suggest that epigenetic treatments with TSA and 5-aza may modulate CRISP3 expression,warranting further research to elucidate its regulatory mechanisms and potential as a prognostic biomarker.

关 键 词：cervical cancer uterine cervical carcinoma CRISP3 biomarkers in cancer PROGNOSIS 

分 类 号：R73[医药卫生—肿瘤]