花姜酮通过miRNA-1-3p/E2F8通路对宫颈癌细胞化疗敏感性的影响  

The effect of zingerone on the chemotherapy sensitivity of cervical cancer cells through the miRNA-1-3p/E2F8 pathway

作　　者：胡贝茜 曹雷 杨嘉安 朱虹[1] HU Beiqian;CAO Lei;YANG Jia’an;ZHU Hong(Harbin Medical University,Harbin,Heilongjiang 150001,China)

机构地区：[1]哈尔滨医科大学,黑龙江哈尔滨150001

出　　处：《中国优生与遗传杂志》2024年第12期2468-2473,共6页Chinese Journal of Birth Health & Heredity

基　　金：2023年国家自然科学基金青年科学基金(82304250);2022年黑龙江省哲学社会科学研究规划项目(22GLB125)。

摘　　要：目的探究花姜酮通过miRNA-1-3p/E2F8通路对宫颈癌细胞化疗敏感性的影响。方法将宫颈癌细胞分别以化疗/转染/花姜酮为单一变量进行分组,经流式细胞术等检测细胞功能影响情况,实时定量PCR(RT-qPCR)及蛋白质免疫印迹(WB)分别检测miRNA-1-3p和E2F8蛋白的表达情况。结果与化疗+miRNA-1-3p转染组比较,花姜酮+miRNA-1-3p转染+化疗组细胞活力下降,凋亡率提升(P<0.05),miRNA-1-3p表达量提升(P<0.05),E2F8蛋白表达量降低(P<0.05)。结论miRNA-1-3p/E2F8通路参与调控宫颈癌细胞的增殖及凋亡;花姜酮通过miRNA-1-3p/E2F8通路参与调控宫颈癌细胞的增殖、凋亡及对紫杉醇和顺铂联合化疗的敏感性。Objective To investigate the effect of zingerone on the chemotherapy sensitivity of cervical cancer cells through the miRNA-1-3p/E2F8 pathway.Methods Cervical cancer cells were divided into groups with chemotherapy/transfection/zingerone as single variables.Cell function was detected by flow cytometry,and the expression of miRNA-1-3p and E2F8 protein was detected by real-time quantitative PCR(RT-qPCR)and Western blot(WB),respectively.Results Compared with the chemotherapy+miRNA-1-3p transfection group,the zingerone+miRNA-1-3p transfection+chemotherapy group showed decreased viability,increased apoptosis rate(P<0.05),increased miRNA-1-3p expression(P<0.05),and decreased E2F8 protein expression(P<0.05).Conclusion The miRNA-1-3p/E2F8 pathway is involved in the regulation of proliferation and apoptosis of cervical cancer cells.Zingerone regulates the proliferation,apoptosis,and sensitivity to paclitaxel and cisplatin combined chemotherapy of cervical cancer cells through the miRNA-1-3p/E2F8 pathway.

关 键 词：花姜酮 miRNA-1-3p/E2F8通路 宫颈癌 化疗敏感性 

分 类 号：R73[医药卫生—肿瘤]