灯盏花素调节Akt/MDM2/p53信号通路对乳腺癌细胞发生发展的影响  

Effects of breviscapine on the occurrence and development of breast cancer cells by regulating Akt/MDM2/p53 signaling pathway

作　　者：李静[1] 杨庚武[1] 李颖[2] 王志芬[1] 刘峥[1] LI Jing;YANG Gengwu;LI Ying;WANG Zhifen;LIU Zheng(Three Departments of Cancer,Handan Central Hospital,Handan,Hebei 056000,China;Department of Oncology,the Fourth Hospital of Hebei Medical University,Shijiazhuang,Hebei 050000,China)

机构地区：[1]邯郸市中心医院肿瘤内三科,河北邯郸056000 [2]河北医科大学第四医院肿瘤内科,河北石家庄050000

出　　处：《中国优生与遗传杂志》2024年第12期2474-2479,共6页Chinese Journal of Birth Health & Heredity

基　　金：河北省2019年度医学科学研究课题计划(20190193)。

摘　　要：目的探讨灯盏花素(BRE)调节Akt/MDM2/p53信号通路对人乳腺癌细胞发生发展的影响。方法将MCF-7细胞和MDA-MB-231细胞分别分为:Ctrl组、低浓度BRE组(BRE-L,25μmol/L)、中浓度BRE组(BRE-M,50μmol/L)和高浓度BRE组(BRE-H,100μmol/L);CCK-8法、集落形成实验检测乳腺癌细胞增殖情况;划痕实验检测乳腺癌细胞迁移;Transwell实验检测乳腺癌细胞侵袭;Western blot测定MDM2和p53蛋白表达水平以及p-Akt磷酸化水平;流式细胞术检测乳腺癌细胞凋亡情况。结果与Ctrl组相比,BRE-L(25μmol/L)、BRE-M(50μmol/L)、BRE-H(100μmol/L)组中乳腺癌细胞存活率、集落形成数量、划痕愈合率和侵入率均显著降低(P<0.05),细胞凋亡率显著增加(P<0.05);BRE显著下调乳腺癌细胞中MDM2和p53蛋白表达水平(P<0.05)以及Akt磷酸化水平(P<0.05)。结论BRE可能通过下调Akt/MDM2/p53信号通路中关键蛋白的表达以及p-Akt磷酸化,减弱乳腺癌细胞增殖、迁移和侵袭能力,促进乳腺癌细胞凋亡。Objective To investigate the effects of breviscapine(BRE)on the occurrence and development of human breast cancer cells by regulating Akt/MDM2/p53 signaling pathway.Methods MCF-7 cells and MDA-MB-231 cells were separated into Ctrl group,low concentration BRE group(BRE-L,25μmol/L),medium concentration BRE group(BRE-M,50μmol/L),and high concentration BRE group(BRE-H,100μmol/L),respectively.CCK-8 method and colony forming test were applied to detect the proliferation of breast cancer cells.Scratch test was used to measure breast cancer cell migration.Transwell experiment was used to measure the invasion of breast cancer cells.Western blot was applied to determine the expression levels of MDM2 and p53 proteins,and the phosphorylation level of p-Akt.Flow cytometry was used to detect apoptosis in breast cancer cells.Results Compared with Ctrl group,the cell survival rate,colony forming number,scratch healing rate,invasion rate of breast cancer cells in BRE-L(25μmol/L),BRE-M(50μmol/L)and BRE-H(100μmol/L)groups were obviously reduced(P<0.05),and the apoptosis rate was significantly increased(P<0.05).BRE obviously decreased the expression levels of MDM2 and p53 proteins and the phosphorylation of p-Akt in breast cancer cells(P<0.05).Conclusion BRE may reduce the proliferation,migration and invasion of breast cancer cells and promote apoptosis of breast cancer cells by down-regulating the expression of key proteins in Akt/MDM2/p53 signaling pathway and the phosphorylation of p-Akt.

关 键 词：灯盏花素 Akt/MDM2/p53信号通路 乳腺癌细胞 

分 类 号：R73[医药卫生—肿瘤]