Controllable synthesis of hydrogen-bonded organic framework encapsulated enzyme for continuous production of chiral hydroxybutyric acid in a two-stage cascade microreactor  

作　　者：Danyang Zhao Qiangqiang Xue Yujun Wang Guangsheng Luo 

机构地区：[1]State Key Lab of Chemical Engineering,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China

出　　处：《Chinese Journal of Chemical Engineering》2025年第1期175-184,共10页中国化学工程学报(英文版)

基　　金：supported by the National Key Research and Development Program of China(2019YFA0905100);the National Natural Science Foundation of China(21991102,22378227).

摘　　要：Constructing a framework carrier to stabilize protein conformation,induce high embedding efficiency,and acquire low mass-transfer resistance is an urgent issue in the development of immobilized enzymes.Hydrogen-bonded organic frameworks(HOFs)have promising application potential for embedding enzymes.In fact,no metal involvement is required,and HOFs exhibit superior biocompatibility,and free access to substrates in mesoporous channels.Herein,a facile in situ growth approach was proposed for the self-assembly of alcohol dehydrogenase encapsulated in HOF.The micron-scale bio-catalytic composite was rapidly synthesized under mild conditions(aqueous phase and ambient temperature)with a controllable embedding rate.The high crystallinity and periodic arrangement channels of HOF were preserved at a high enzyme encapsulation efficiency of 59%.This bio-composite improved the tolerance of the enzyme to the acid-base environment and retained 81%of its initial activity after five cycles of batch hydrogenation involving NADH coenzyme.Based on this controllably synthesized bio-catalytic material and a common lipase,we further developed a two-stage cascade microchemical system and achieved the continuous production of chiral hydroxybutyric acid(R-3-HBA).

关 键 词：Hydrogen-bonded organic framework Immobilized enzyme Synthesis Chiral hydroxycarboxylic acid BIOCATALYSIS Nanostructure 

分 类 号：X70[环境科学与工程—环境工程]