慢加急性肝衰竭患者Toll样受体3触发后树突状细胞分泌细胞因子的变化分析  

Analysis of Changes in Cytokine Secretion by Dendritic Cells Following Toll-like Receptor 3 Activation in Patients with Acute-on-Chronic Liver Failure

作　　者：施青青 Shi Qingqing(Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 201600,China)

机构地区：[1]上海交通大学医学院附属松江医院,上海201600

出　　处：《中外医药研究》2025年第6期126-128,共3页JOURNAL OF CHINESE AND FOREIGN MEDICINE AND PHARMACY RESEARCH

基　　金：上海市松江区科学技术委员会科技攻关项目(医药卫生类)(编号:18sjgg9943)。

摘　　要：目的:分析慢加急性肝衰竭患者Toll样受体3(TLR3)触发后树突状细胞(DC)分泌细胞因子的变化。方法:选取2019年1月—2021年12月上海交通大学医学院附属松江医院收治的86例慢加急性肝衰竭患者作为观察组,并选取同时间段收治的86例慢性乙型肝炎患者作为对照组。两组患者均采集血液样本并分离单核细胞,经实验室培养DC,经聚肌胞苷酸刺激后,采用实时荧光定量聚合酶链式反应法检测TLR3分泌核因子κB(NF-κB)及干扰素调节因子3(IRF3)水平,并采用流式细胞仪检测DC分泌炎性细胞因子[白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)],比较两组NF-κB信使核糖核酸(mRNA)、IRF3 mRNA,以及炎性细胞因子表达水平的差异。结果:观察组NF-κB mRNA高于对照组,IRF3 mRNA低于对照组(P<0.05);观察组DC分泌IL-6、TNF-α水平高于对照组,IL-8、IL-10水平低于对照组(P<0.05)。结论:与慢性乙型肝炎患者相比,慢加急性肝衰竭患者TLR3触发后DC分泌NF-κB、IL-6、TNF-α升高,IRF3、IL-8、IL-10下降。Objective:To analyze the changes in cytokine secretion by dendritic cells(DC)following Toll-like receptor 3(TLR3)activation in patients with acute-on-chronic liver failure(ACLF).Methods:From January 2019 to December 2021,86 patients with ACLF were selected as the observation group,and 86 patients with chronic hepatitis B(CHB)were selected as the control group.Blood samples were collected from both groups,and monocytes were isolated.DC were cultured in vitro and stimulated with polyinosinic-polycytidylic acid.The levels of nuclear factor-κB(NF-κB)and interferon regulatory factor 3(IRF3)secreted by TLR3 were detected using real-time quantitative polymerase chain reaction.The secretion of inflammatory cytokines[interleukin-6(IL-6),interleukin-8(IL-8),interleukin-10(IL-10),and tumor necrosis factor-α(TNF-α)]by DC was measured using flow cytometry.The differences in NF-κB mRNA,IRF3 mRNA,and inflammatory cytokine expression levels were compared between the two groups.Results:The NF-κB mRNA level in the observation group was higher than that in the control group,while the IRF3 mRNA level was lower(P<0.05).The secretion of IL-6 and TNF-αby DC in the observation group was higher than that in the control group,whereas the secretion of IL-8 and IL-10 was lower(P<0.05).Conclusion:Compared with CHB patients,ACLF patients exhibited increased secretion of NF-κB,IL-6,and TNF-α,and decreased secretion of IRF3,IL-8,and IL-10 by DCs following TLR3 activation.

关 键 词：慢加急性肝衰竭 慢性乙型肝炎 TOLL样受体3 树突状细胞 细胞因子 炎性因子 

分 类 号：R575.3[医药卫生—消化系统]