肝细胞癌VOPP1表达及其与预后的关系  

Expression of VOPP1 in hepatocellular carcinoma and its relationship with prognosis

作　　者：陈晓鹏 王佳妮[2] 练庆海 杨九妹 Chen Xiaopeng;Wang Jiani;Lian Qinghai;Yang Jiumei(Third Clinical Medical College of Ningxia Medical University,Yinchuan 750002,China;Department of Breast and Thyroid Surgery,the Trird Affliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Institute of Vaccine,the Trird Affliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Medical Experiment Center,Guangdong Second Provincial General Hospital,Guangzhou 510317,China)

机构地区：[1]宁夏医科大学第三临床医学院,银川750002 [2]中山大学附属第三医院乳腺与甲状腺外科,广州510630 [3]中山大学附属第三医院疫苗研究所,广州510630 [4]广东省第二人民医院医学实验中心,广州510317

出　　处：《中华肝脏外科手术学电子杂志》2024年第6期876-882,共7页Chinese Journal of Hepatic Surgery(Electronic Edition)

基　　金：广东省自然科学基金(2017A030313858)

摘　　要：目的探讨囊泡过表达肿瘤促生存蛋白1(VOPP1)在肝细胞癌(肝癌)中的表达及其与患者预后关系。方法回顾性分析2008年2月至2015年8月在中山大学附属第三医院行根治性切除术的90例肝癌患者临床病理资料。患者均签署知情同意书,符合医学伦理学规定。其中男81例,女9例;年龄27~74岁,中位年龄53岁;TNM分期Ⅰ期11例,Ⅱ期29例,Ⅲ期26例,Ⅳ期2例;采用免疫组化法检测肝癌组织及癌旁组织VOPP1表达,分析VOPP1表达与临床病理指标之间的关系。另外收集10例患者肝癌组织及癌旁组织,采用qRT-PCR检测VOPP1 mRNA表达水平。生存分析采用Kaplan-Meier法和Log-rank检验,Cox回归模型分析患者预后的独立危险因素。结果qRT-PCR检测显示,肝癌组织VOPP1 mRNA平均相对表达量为(4.91±1.98)×10^(-3),明显高于配对癌旁组织的(0.39±0.12)×10^(-3)(t=2.284,P<0.05)。免疫组化法检测显示,肝癌组织VOPP1阳性表达率为52%(47/90),明显高于癌旁组织的28%(25/90)(χ^(2)=11.204,P<0.05)。肝癌组织VOPP1高表达与组织学分级相关(χ^(2)=9.373,P<0.05)。生存分析显示,VOPP1高表达组和低表达组肝癌根治性切除术患者的中位无复发生存期(RFS)分别为17、50个月,VOPP1高表达组患者的RFS明显低于VOPP1低表达组(χ^(2)=7.769,P<0.05)。Cox回归分析显示,T3分期(HR=3.649,95%CI:1.278~10.420)、T4分期(HR=6.157,95%CI:1.297~29.235)和VOPP1蛋白高表达(HR=1.866,95%CI:1.069~3.258)均是影响患者术后RFS的独立危险因素(P<0.05)。结论VOPP1在肝癌组织中高表达,其表达水平与患者预后呈负相关,且是肝癌预后的独立危险因素,可作为一个有价值的预后预测新指标。Objective To investigate the expression of vesicular over-expressed in cancer prosurvival protein 1(VOPP1)in hepatocellular carcinoma(HCC)and its relationship with clinical prognosis of HCC patients.Methods Clinicopathological data of 90 HCC patients who underwent radical resection in the Third Affiliated Hospital of Sun Yat-sen University from February 2008 to August 2015 were retrospectively analyzed.The informed consents of all patients were obtained and the local ethical committee approval was received.Among them,81 patients were male and 9 female,aged 27-74 years,with a median age of 53 years.11 cases were classified as TNM stageⅠ,29 cases of TNM stageⅡ,26 cases of TNM stageⅢand 2 cases of TNM stageⅣ.The expression level of VOPP1 in HCC and adjacent tissues was determined by immunohistochemical staining,and the relationship between VOPP1 expression and clinicopathological parameters was analyzed.In addition,the expression level of VOPP1 mRNA in the cancer and adjacent tissues of 10 HCC patients was detected by qRT-PCR.Survival analysis was conducted by Kaplan-Meier method and Log-rank test.The independent risk factors of clinical prognosis were assessed by Cox regression model.Results qRT-PCR showed that the average relative expression of VOPP1 mRNA in HCC tissues was(4.91±1.98)×10^(-3),significantly higher than(0.39±0.12)×10^(-3)in paired adjacent tissues(t=2.284,P<0.05).Immunohistochemical staining showed that the positive expression rate of VOPP1 in HCC was 52%(47/90),significantly higher than 28%(25/90)in adjacent tissues(χ^(2)=11.204,P<0.05).The high expression of VOPP1 in HCC tissues was correlated with histological grade(χ^(2)=9.373,P<0.05).Survival analysis showed that the median recurrence-free survival(RFS)of HCC patients with high and low expression of VOPP1 after radical resection was 17 and 50 months,respectively.The RFS of patients with high expression of VOPP1 was significantly lower than that of their counterparts with low expression of VOPP1(χ^(2)=7.769,P<0.05).Cox regression

关 键 词：癌 肝细胞 预后 VOPP1 肿瘤标记物 

分 类 号：R735.7[医药卫生—肿瘤]