杭州市2~4年级学生睡眠时间对肠道菌群结构影响的研究  

Research on the effect of sleep duration on the gut microbiota composition of pupils from grades 2 to 4 in Hangzhou City

作　　者：吴彦[1] 王兵 皮雄娥[2] 谢璐 金慧[1] 考庆君[1] 赵刚[1] WU Yan;WANG Bing;PI Xiong'e;XIE Lu;JIN Hui;KAO Qingjun;ZHAO Gang(Hangzhou Center for Disease Control and Prevention,Hangzhou Health Supervision Institution,Hangzhou,Zhejiang 310021,China;不详)

机构地区：[1]杭州市疾病预防控制中心杭州市卫生监督所,浙江杭州310021 [2]浙江省农业科学院植物保护与微生物研究所 [3]杭州师范大学

出　　处：《中国预防医学杂志》2025年第2期211-216,共6页Chinese Preventive Medicine

基　　金：杭州市科技发展计划项目(202004A20)。

摘　　要：目的分析杭州市2~4年级小学生的肠道菌群组成特征及睡眠时间对肠道菌群组成的影响。方法纳入2022年3—5月杭州市2~4年级小学生156名,应用16S rRNA基因测序技术和生物信息学分析,结合对睡眠时间的问卷调查,对研究对象的粪便细菌DNA进行测序分析。结果将156名调查对象分为每天睡眠时长<9 h组和≥9 h组,阿尔法多样性指数分析显示,组间Shannon指数差异有统计学意义(t=-3.47,P<0.01),Chao指数差异无统计学意义(t=-0.90,P=0.37)。基于Bray-Curtis距离的主坐标分析图显示,两组的样本分布有明显分离(R^(2)=0.01,P<0.05)。每天睡眠时间<9 h组和≥9 h组的放线菌门、双歧杆菌属、毛螺菌科丰度比较差异有统计学意义(t=11.21、36.53、23.08,P<0.05)。结合组间菌属丰度的秩和检验和线性判别差异贡献分析结果显示,睡眠时长<9 h组显著偏高的只有双歧杆菌属,而睡眠时长≥9 h组偏高的主要有毛螺菌科未分型属、毛螺菌科nk4a136菌属、丁酸球菌属、克里斯滕森菌科R-7菌属和韦荣球菌属5个菌属。结论睡眠时长能影响儿童青少年的肠道菌群结构和分布,充足的睡眠能提高肠道菌群多样性,尤其能增加有益菌群的丰度,保证充足的睡眠时间对促进儿童青少年身心健康发展至关重要。Objective To investigate the characteristics of gut microbiota composition of pupils from grades 2 to 4in Hangzhou city and explore the effect of sleep duration on it.Methods From March to May 2022,156 primary school students from grades 2 to 4 in Hangzhou City were included in the study.16S rRNA gene sequencing technology and bioinformatics analysis were used to analyze the fecal bacterial DNA of the study subjects,combined with a questionnaire survey on sleep duration.Results The 156 subjects were divided into two groups according to the daily sleep duration:those sleeping less than 9 hours per day and those sleeping 9 hours or more.The analysis of the Alpha diversity index between groups showed that the Shannon index had a significant difference between groups(t=-3.47,P<0.01),while the Chao index had no significant difference between groups(t=-0.90,P=0.37).The principal coordinate analysis(PCoA)graph based on Bray-Curtis distance showed that the sample distributions of the two groups were significantly separated(R^(2)=0.01,P<0.05).The abundance of Actinobacteria,Bifidobacterium and Lachnospiraceae between the two groups was significantly different(t=11.21,36.53,23.08,P<0.05).Linear discriminant analysis Effect Size(LEfSe)analysis(LDA>3,P<0.05)showed that only Bifidobacterium in the group sleeping for less than 9 hours was significantly higher,while 5 genera such as unclassified_f_Lachnospiraceae,Lachnospiraceae_NK4A136_group,Butyricicoccus,Christensenellaceae_R_group and Veillonella were significantly higher in the group sleeping for 9 hours and above.Conclusions Sleep duration can affect the structure and distribution of gut microbiota in children and adolescents.Adequate sleep may improve the diversity of gut microbiota,especially the abundance of beneficial microbiota.In order to promote the healthy development of children and adolescents,it is very important to ensure adequate sleep duration.

关 键 词：睡眠时间 肠道菌群 多样性 小学生 16S rRNA测序 

分 类 号：R723.14[医药卫生—儿科]