Development of a porcine model of skin and soft-tissue infection caused by Staphylococcus aureus,including methicillin-resistant strains suitable for testing topical antimicrobial agents  

作　　者：Filip Raška Břetislav Lipový Šárka Kobzová LukášVacek Rea Jarošová Dominika Kleknerová Katarína Matiašková Peter Makovický Monika Vícenová Edita Jeklová Roman Pantůček Martin Faldyna Lubomír Janda 

机构地区：[1]Department of Burns and Plastic Surgery,Faculty of Medicine,Institution Shared with University Hospital Brno,Masaryk University,Brno,Czech Republic [2]Department of Burns Medicine,Third Faculty of Medicine,Charles University and University Hospital Kralovske Vinohrady,Vinohrady,Prague,Czech Republic [3]CEITEC-Central European Institute of Technology,Brno University of Technology,Brno,Czech Republic [4]Department of Infectious Diseases and Preventive Medicine,Veterinary Research Institute,Brno,Czech Republic [5]Department of Biochemistry,Faculty of Science,Masaryk University,Brno,Czech Republic [6]Department of Microbiology,St.Anne's University Hospital Brno and Faculty of Medicine,Masaryk University,Brno,Czech Republic [7]Department of Morphology,Physiology and Animal Genetics,Faculty of AgriSciences,Mendel University in Brno,Brno,Czech Republic [8]Department of Histology and Embryology,Faculty of Medicine,University of Ostrava,Ostrava,Czech Republic [9]Department of Experimental Biology,Faculty of Science,Masaryk University,Brno,Czech Republic

出　　处：《Animal Models and Experimental Medicine》2025年第3期544-557,共14页动物模型与实验医学(英文)

基　　金：Supported by the Ministry of Health of the Czech Republic,Grant/Award Number:NU22-05-00475 and NV19-05-00214。

摘　　要：Background:In view of the ever-increasing representation of Staphylococcus spp.strains resistant to various antibiotics,the development of in vivo models for evaluation of novel antimicrobials is of utmost importance.Methods:In this article,we describe the development of a fully immunocompetent porcine model of extensive skin and soft tissue damage suitable for testing topical anti-microbial agents that matches the real clinical situation.The model was developed in three consecutive stages with protocols for each stage amended based on the results of the previous one.Results:In the final model,10 excisions of the skin and underlying soft tissue were created in each pig under general anesthesia,with additional incisions to the fascia performed at the base of the defects and immediately inoculated with Staphylococcus aureus suspension.One pig was not inoculated and used as the negative control.Subsequently,the bandages were changed on Days 4,8,11,and 15.At these time points,a filter paper imprint technique(FPIT)was made from each wound for semi-quantitative microbiological evaluation.Tissue samples from the base of the wound together with the adjacent intact tissue of three randomly selected defects of each pig were taken for microbiological,histopathological,and molecular-biological examination.The infection with the inoculated S.aureus strains was sufficient during the whole experiment as confirmed by both FPIT and from tissue samples.The dynamics of the inflammatory markers and clinical signs of infection are also described.Conclusions:A successfully developed porcine model is suitable for in vivo testing of novel short-acting topical antimicrobial agents.

关 键 词：animal model antimicrobial agents porcine model skin and soft-tissue infection(SSTI) Staphylococcus aureus wound infection 

分 类 号：R36[医药卫生—病理学]