A surgical orthotopic xenograft approach with immune response for colorectal cancer research  

作　　者：Xiaoying Hou Xiaoxuan Li Qian Fang Yufei Deng Haiping Wang Binlian Sun Chengliang Zhang Hongzhi Du Yuchen Liu 

机构地区：[1]Cancer Institute,School of Medicine,Jianghan University,Wuhan,China [2]Hubei Key Laboratory of Cognitive and Affective Disorders,Jianghan University,Wuhan,China [3]Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China [4]Center of Traditional Chinese Medicine Modernization for Liver Diseases,Hubei University of Chinese Medicine,Wuhan,China

出　　处：《Animal Models and Experimental Medicine》2025年第3期558-567,共10页动物模型与实验医学(英文)

基　　金：Research Fund of Jianghan University,Grant/Award Number:2021jczx-002;Natural Science Foundation of Hubei Province,Grant/Award Number:2024AFB941 and 2024AFD241;Wuhan Science and Technology Project,Grant/Award Number:2023010201020448;National Natural Science Foundation of China,Grant/Award Number:82204416。

摘　　要：The high morbidity and mortality of colorectal cancer(CRC)is a major challenge in clinical practice.Although a series of alternative research models of CRC have been developed,appropriate orthotopic animal models that reproduce the specific clinical response as well as pathophysiological immune features of CRC are still lacking.In the current study,we constructed a CRC orthotopic xenograft model by implanting the tumor tubes at the colorectum of mice and monitored the model development using bioluminescence imaging.This model successfully recapitulates the clinical chemo-therapy efficacy,including reduced total flux,tumor weight,and the expression of Ki67 after treatment of the first-line chemotherapy regime of CRC(FOLFOX:oxaliplatin and 5-fluorouracil/calcium folinate).The model also reproduced the immunosup-pressive effect of FOLFOX,indicated by decreased infiltration of macrophages and increased Treg cells in tumor.Additionally,the orthotopic xenograft approach may be applied in immunodeficient NCG/NSG mice for constructing patient-derived xeno-grafts,and being used in clinical precision medicine and drug evaluation.We believe the current model is a successful surgical orthotopic xenograft approach for cancer research and deserves to be popularized,which will provide a convenient and efficient platform for in-depth mechanism exploration of CRC and preclinical drug evaluation.

关 键 词：animal model colorectal cancer orthotopic tumor model tumor immunology 

分 类 号：R73[医药卫生—肿瘤]