基于m5C修饰相关基因的肺腺癌预后模型的建立与验证  

Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related Genes

作　　者：杨帆 王浓燕 方蒙 章莹姣 胡海燕 方鹏 YANG Fan;WANG Nongyan;FANG Meng;ZHANG Yingjiao;HU Haiyan;FANG Peng(Department of General Medicine,No.903 Hospital of PLA Joint Logistic Support Force(Affiliated Xihu Hospital of Hangzhou Medical College),Hangzhou 310012,China)

机构地区：[1]联勤保障部队第九〇三医院(杭州医学院附属西湖医院)全科医学科,杭州310012

出　　处：《肿瘤防治研究》2025年第3期208-216,共9页Cancer Research on Prevention and Treatment

基　　金：浙江省医药卫生科技计划项目(2021KY946);第903医院自主科研项目(YQ202306);浙江省教育厅一般科研项目(Y202456549)。

摘　　要：目的基于m5C修饰相关基因构建并验证肺腺癌(LUAD)预后模型,并探讨其临床应用价值。方法基于TCGA和GSE30219、GSE31210、GSE50081等LUAD数据集资料,通过单因素Cox、Lasso、多因素Cox回归分析筛选预后相关基因并构建模型,通过绘制Kaplan-Meier曲线、ROC曲线及Cox回归观察模型的稳健性和预后性能,并探讨模型与临床病理特征的相关性。结果构建了由CDK1、CDKN1A、NOP2、RRM2、TCL6、TLR8、TRDMT1、YTHDF2等8个m5C修饰相关基因组成的预后模型。风险评分是LUAD患者预后的独立危险因素,其联合T分期和N分期的列线图能更准确地预测患者的预后。高风险组患者中巨噬细胞、CD4^(+)/CD8^(+)T细胞等浸润显著减少。LUAD组织中风险评分显著高于正常组织,且与T分期、N分期正相关。吸烟、EGFR野生型患者的风险评分显著升高。结论基于m5C修饰相关基因构建的预后模型,对预测LUAD患者的预后评估具有较好的准确性和稳定性,且与患者的临床特征、驱动基因突变、免疫浸润等密切相关,可为LUAD的治疗与预后评估提供潜在依据。Objective To construct a prognostic model of lung adenocarcinoma(LUAD)based on m5C modification-related genes and to explore its clinical value.Methods Based on the LUAD data in TCGA,GSE30219,GSE31210,and GSE50081 cohorts,prognosis-related m5C modification-related genes were screened,and the prognostic model was constructed by using univariate Cox,Lasso,and multivariate Cox regression analyses.Kaplan-Meier curve,ROC curve,and Cox regression were used to observe the robustness and prognostic performance of the model.The correlation between the prognostic model and clinicopathologic features was further explored.Results A prognostic model consisting of eight m5C modification-related genes,including CDK1,CDKN1A,NOP2,RRM2,TCL6,TLR8,TRDMT1,and YTHDF2,was constructed.Risk score was an independent risk factor for the prognosis of patients with LUAD,and it is combined with age,T stage,and N stage to constitute a nomogram which can accurately predict the prognosis of patients.The infiltration of macrophages and CD4^(+)/CD8^(+)T cells was significantly reduced in high-risk patients.The risk score in LUAD tissues was significantly higher than that in normal tissues and was positively correlated with T stage and N stage.The risk score of smoking and EGFR wild-type patients was higher than that of non-smoking and EGFR-mutant patients.Conclusion The prognostic model constructed based on m5C modification-related genes has shown good accuracy and stability in predicting the prognosis of patients with LUAD,and it is closely related to clinical features,driver gene mutations,and immune infiltration,which can provide a potential basis for the treatment and prognostic assessment of LUAD.

关 键 词：肺腺癌 甲基化修饰 m5C 预后模型 风险评分 列线图 

分 类 号：R734.2[医药卫生—肿瘤]