蛋白质组学前处理技术的研究进展  

Advances in Proteomics Pre-processing Techniques

作　　者：杨玉萍[1] 黄睿 YANG Yuping;HUANG Rui(Beijing Key Laboratory of Gene Resources and Molecular Development,College of Life Sciences,Beijing Normal University,Beijing 100875,China;Key Lab of Stomatology of State Ethnic Affairs Commission,Northwest Minzu University,Lanzhou 730030,China;Key Laboratory of Environmental Ecology and Population Health in Northwest Minority Areas,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]北京师范大学抗性基因资源与分子发育北京市重点实验室,北京100875 [2]西北民族大学口腔医学国家民委重点实验室,甘肃兰州730030 [3]西北民族地区环境生态与人群健康国家民委重点实验室,甘肃兰州730030

出　　处：《西北民族大学学报(自然科学版)》2025年第1期17-24,共8页Journal of Northwest Minzu University(Natural Science)

摘　　要：蛋白质组学前处理是蛋白质组学研究的关键,直接影响到后续分析的准确性和可靠性.文章综述了蛋白质组学前处理各环节的研究进展,包括样品制备、蛋白质提取、蛋白质消化、肽分级和肽纯化等.详细介绍了各种技术方法的原理、优缺点以及应用范围,阐述了采用新型蛋白酶组合的酶解方法及样本特异数据库的数据解析方法,并对未来的发展趋势进行了展望.The pretreatment of proteomics is the key to proteomics research and directly affects the accuracy and reliability of subsequent analyses.This article reviews the progress in all aspects of proteomics pretreatment,including the progress in sample preparation,protein extraction,protein digestion,peptide fractionation and peptide purification.It elaborates on the principles,advantages and disadvantages as well as the application ranges of various technical methods,expounds the enzymatic hydrolysis methods using new protease combinations and the data analysis methods for sample-specific databases,and also looks forward to the future development trends.

关 键 词：蛋白质组学 前处理 样品制备 蛋白质消化 肽纯化 

分 类 号：Q789[生物学—分子生物学]