miRNA-21-5p targeting PTEN to regulate PI3K/Akt/mTOR pathway in retinal pigment epithelial cell photodamage  

作　　者：Juan Li Ruo-Di Shi Qing Li Chen Xu Yang Yu 

机构地区：[1]Peking University First Hospital Ningxia Women and Children’s Hospital(Ningxia Hui Autonomous Region Maternal and Child Health Hospital),Yinchuan 750011,Ningxia Hui Autonomous Region,China [2]School of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan 750004,Ningxia Hui Autonomous Region,China [3]Department of Functional Examination,Gynecology and Obstetrics,General Hospital of Ningxia Medical University,Yinchuan 750004,Ningxia Hui Autonomous Region,China [4]Key Laboratory of Modernization of Ethnic Minority Medicine,Ministry of Education,Yinchuan 750004,Ningxia Hui Autonomous Region,China

出　　处：《International Journal of Ophthalmology(English edition)》2025年第4期575-581,共7页国际眼科杂志(英文版)

摘　　要：AIM:To highlight the importance of microRNA(miRNA)-21-5p in directing the phosphatase and tensin homolog(PTEN)gene to control the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)pathway in retinal pigment epithelial(RPE)cells in humans subjected to photodamage.METHODS:Human adult RPE cell line-19(ARPE-19)was cultured in vitro and randomly divided into control,damage,overexpression,negative,and PI3K/Akt blocker groups to establish a photodamage model of ARPE-19 cells.The models were subjected to 24h of light exposure,after which the corresponding indices were detected.The cell counting kit-8 assay quantified cell viability,while flow cytometry determined apoptosis rates.The miRNA-21 mimics and miRNA mimic NC were transfected into ARPE-19 cells using a transient transfection technique.Quantitative reverse transcription polymerase chain reaction(SYBR Green)and Western blotting analyzed expression levels of miRNA-21-5p,PTEN,p-PI3K/PI3K,p-mTOR/mTOR,and p-Akt/Akt.Statistical analyses comprised one-way analysis of variance and the Student-Newman-Keuls test for multiple group comparisons.RESULTS:The photodamage group demonstrated reduced cell survival rates than the control group(P<0.01).The overexpression group exhibited higher cell survival rates than the injury group(P<0.01).The negative group showed no difference in viability(P>0.05).The PI3K/Akt blocker group demonstrated lower cell viability,compared with the overexpression group(P<0.01).CONCLUSION:miRNA-21-5p significantly increases ARPE-19 cell survival after photodamage and inhibits lightinduced ARPE-19 cell apoptosis,suggesting that it may play a protective role in RPE by activating the PI3K/Akt/mTOR pathway while downregulating PTEN expression.

关 键 词：retinal pigment epithelial cell PHOTODAMAGE apoptosis PI3K/Akt/mTOR signaling pathway miRNA-21-5p 

分 类 号：R73[医药卫生—肿瘤]