Inhibition of IGF-1Rαaffects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro  

作　　者：Qi-You Li Xiao-Ling Tan Hai-Wei Xu Yu-Xiao Zeng Xiao-Yong Huang 

机构地区：[1]Southwest Hospital/Southwest Eye Hospital,Third Military Medical University(Army Medical University),Chongqing 400038,China [2]Key Lab of Visual Damage and Regeneration&Restoration of Chongqing,Chongqing 400038,China [3]Department of Frigid Zone Medicine,College of High Altitude Military Medicine,Third Military Medical University(Army Medical University),Chongqing 400038,China

出　　处：《International Journal of Ophthalmology(English edition)》2025年第4期582-589,共8页国际眼科杂志(英文版)

摘　　要：AIM:To explore the impact of insulin-like growth factor-1 receptorα(IGF-1Rα)on the differentiation fate of optic-cupderived retinal stem cells(OC-RSCs)into retinal ganglion cells(RGCs)in vitro.METHODS:OC-RSCs were isolated from optic cups of rats on embryonic day 12.5,and high-purity OC-RSCs were obtained by conditioned culture and passage.Differentiation of OC-RSCs into RGCs under different serum concentrations was examined using flow cytometry,and the serum concentration with high interference with differentiation ratio was selected.Furthermore,the effect of blocking IGF-1Rαon the differentiation of OC-RSCs into RGCs was analyzed through immunocytochemistry and Western blotting.RESULTS:Immunohistochemical analysis revealed IGF-1Rαwas highly expressed in rat embryos at day 12.5.OC-RSCs were isolated and purified,and high-purity OCRSCs were obtained.When 2.5%serum was administered,the ratio of differentiated RGCs(Thy-1.1 positive)decreased significantly,and the results of immunoblotting also confirmed the blockade of IGF-1Rαreduced Thy-1.1 protein expression.CONCLUSION:IGF-1Rαblocking can reduce the differentiation of OC-RSCs into RGCs.

关 键 词：insulin-like growth factor-1 receptorα retinal ganglion cell rat optic cup retinal stem cells differentiation fate 

分 类 号：R73[医药卫生—肿瘤]