血清肿瘤标志物联合miR-412、miR-210在非小细胞肺癌患者中的诊断价值与预测价值分析  

Diagnostic value and predictive value of serum tumor markers combined with miR-412 and miR-210 in patients with non-small cell lung cancer

作　　者：颜刚林 朱洪斌[1] 范小玉 YAN Ganglin;ZHU Hongbin;FAN Xiaoyu(Chaohu Hospital Affiliated to Anhui Medical University,Department of Respiratory and Critical Care Medicine,Chaohu,Anhui 238000,China)

机构地区：[1]安徽医科大学附属巢湖医院,安徽巢湖238000

出　　处：《临床肺科杂志》2025年第4期505-512,519,共9页Journal of Clinical Pulmonary Medicine

基　　金：安徽高校自然科学研究项目(KJ2021A0336)。

摘　　要：目的探讨血清肿瘤标志物联合miR-412、miR-210对非小细胞肺癌(NSCLC)的诊断、预测价值。方法将本机构于2022年1月-2023年3月期间收治的75例NSCLC患者和70例肺部良性病变患者纳入本研究,另选取70例健康志愿者作为健康对照组。分别采用免疫化学法和实时荧光定量PCR法检测血清CEA、miR-210和miR-412水平,并使用Logistic回归分析模型和受试者分析曲线(ROC)分析上述血清指标与NSCLC患者临床资料之间的关系及对NSCLC的诊断效能。结果NSCLC组血清CEA、miR-210、miR-412水平均较肺良性病变组和健康对照组明显偏高(均P<0.05)。肿瘤直径>3cm、低分化、临床分期Ⅲ~Ⅳ期、复发及淋巴结转移患者的血清CEA、miR-210及miR-412水平均较肿瘤直径≤3cm、高中分化、临床分期Ⅰ~Ⅱ期、无复发及淋巴结转移患者明显偏高(均P<0.05)。Logstic回归分析结果显示,血清miR210、miR412及CEA水平偏高均是影响NSCLC发生的独立危险因素(P<0.05)。血清CEA、miR-210及miR-412单独检测诊断NCSLC的AUC分别为0.681(95%CI:0.556~0.807)、0.851(95%CI:0.766~0.936)和0.844(95%CI:0.756~0.932),敏感度分别为62.56%、81.25%和84.38%,特异度分别为72.51%、75.09%和70.06%。联合检测的诊断AUC升高至0.905(95%CI:0.841~0.970,P<0.05)。联合检测的诊断AUC高于血清CEA、miR-210及miR-412单独检测的AUC,(Z=3.110,2587,3.215,P<0.05)。结论NSCLC患者的血清中的CEA、miR-412和miR-210表达水平较高,联合检测对NSCLC的发生具有较高的诊断效能。Objective To explore the diagnostic and predictive value of serum tumor markers combined with miR-412 and miR-210 in non-small cell lung cancer(NSCLC).Methods 75 patients with NSCLC and 70 patients with benign lung lesions admitted to our hospital from January 2022 to March 2023 were included in this study,and 70 healthy volunteers were selected as the healthy control group.Serum CEA,miR-210 and Mir-412 levels were detected by immunochemistry and real-time fluorescence quantitative PCR,respectively.The relationship between the above serum indexes and clinical data of NSCLC patients and their diagnostic performance were analyzed by Logistic regression analysis model and ROC.Results Serum CEA,miR-210 and miR-412 levels in the NSCLC group were significantly higher than those in the lung benign lesion group and the healthy control group.(all P<0.05).Serum CEA,miR-210 and miR-412 levels were significantly higher in patients with tumour diameter>3cm,low differentiation,clinical stageⅢ-Ⅳ,recurrence and lymph node metastasis than in patients with tumour diameter≤3cm,high and medium differentiation,clinical stageⅠ-Ⅱ,and no recurrence and metastasis(all P<0.05).The results of logistic regression analysis showed that high serum miR210,miR412 and CEA levels were independent risk factors affecting the occurrence of NSCLC(P<0.05).The AUC score ratio of serum CEA,miR-210 and miR-412 individually detected for the diagnosis of NCSLC was 0.681(95%CI 0.556-0.807),0.851(95%CI 0.766-0.936)and 0.844(95%CI 0.756-0.932),with sensitivity of 62.56%,81.25%and 84.38%,respectively,and specificity of 72.51%,75.09%and 70.06%,respectively.The diagnostic AUC of the combined test was 0.905(95%CI 0.841~0.970,P<0.05).The diagnostic AUC of combined detection was higher than that of serum CEA,miR-210 and miR-412(Z=3.110,2587,3.214,P<0.05).Conclusion The expression levels of CEA,miR-412 and miR-210 in serum of patients with NSCLC are high,and the combined detection has high diagnostic efficiency for NSCLC.

关 键 词：血清肿瘤标志物 MIR-210 miR-412 非小细胞肺癌 诊断价值 

分 类 号：R734.2[医药卫生—肿瘤]