添加乙酸对Saccharomyces cerevisiae L7增产2,3-丁二醇的机理探究  

Investigation of the mechanism of adding acetate to enhance the production of 2,3-butanediol by Saccharomyces cerevisiae L7

作　　者：姚卫格 刘磊 孙蕊 康杰 葛菁萍[1] YAO Weige;LIU Lei;SUN Rui;KANG Jie;GE Jingping(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education/Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fergineering for Cold Region,Key Laboratory of Microbiology,College of Heilongjiang Province,School of Life Sciences,Heilongjiang University,Harbin 150080,China)

机构地区：[1]黑龙江大学生命科学学院农业微生物技术教育部工程研究中心/黑龙江省寒区植物基因与生物发酵重点实验室/黑龙江省普通高校微生物重点实验室,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2025年第1期56-62,共7页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(32071519);黑龙江省省属高校基本科研业务费项目(2023-KYYWF-1448);黑龙江省生态环境厅生态环境保护科研项目(HST20TR004)。

摘　　要：酿酒酵母(Saccharomyces cerevisiae,S.cerevisiae)是生产应用价值较高且可发酵产生清洁化合物2,3-丁二醇(2,3-butanediol)的重要微生物。添加乙酸可以提高S.cerevisiae发酵产2,3-丁二醇的能力,但其机制尚不清楚。本研究在添加乙酸后对胞内外乙酸、还原力、耐酸相关基因表达量(haa1、msn2和msn4)和2,3-丁二醇合成相关基因(bdh1和ilv2)表达量进行分析。添加乙酸使S.cerevisiae的胞内乙酸含量显著高于胞外乙酸,减小了还原力的变化范围,使还原力趋于平衡,耐酸基因以及2,3-丁二醇合成基因表达上调。添加乙酸会通过影响乙酸代谢、细胞内氧化还原状态以及相关基因的表达完成对2,3-丁二醇的增产调控。本研究为大规模提高2,3-丁二醇的产量奠定了技术支撑与理论基础。Saccharomyces cerevisiae(S.cerevisiae)is an important microorganism which has high application value and can be fermented to produce cleaner compound(2,3-butanediol).Addition of acetate can enhance 2,3-butanediol production infermentation ofS.cerevisiae,but the mechanism is not clear.In this study,intracellular and extracellular acetate,reducing power,expression of acid tolerancerelated genes(haa1,msn2,andmsn4),and expression of 2,3-butanediol synthesis-related genes(bdh1andilv2)were analyzed after addition of acetate.The addition of acetate can significantly increase the intracellular acetate content ofS.cerevisiae,reduce the range of the reducing power and bring the reducing power to equilibrium,and up-regulate the expression of the genes related to acid tolerance as well as the genes related to the synthesis of 2,3-butanediol.Addition of acetate will accomplish the regulation of 2,3-butanediol production increased by affecting acetate metabolism,intracellular redox state,and the expression of related genes.This study lays the technical support and theoretical foundation for the large-scale improvement of 2,3-butanediol production.

关 键 词：酿酒酵母 2 3-丁二醇 合成机制 乙酸调控 

分 类 号：Q815[生物学—生物工程]