土牛膝提取物对α-葡萄糖苷酶和二肽基肽酶-4的抑制作用研究  

The Inhibition ofα-Glucosidase and Dipeptidyl Peptidase-4 Activity by Extracts of Achyranthes aspera L.

作　　者：江圆圆 胡颖[1] 韦珑洁 黄春韵 罗春艳[1] 苏健[1] Jiang Yuanyuan;Hu Ying;Wei Longjie;Huang Chunyun;Luo Chunyan;Su Jian(Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学,广西南宁530200

出　　处：《广东化工》2025年第3期45-48,共4页Guangdong Chemical Industry

基　　金：广西壮瑶药重点实验室(桂科基字[2014]32号);壮瑶药协同创新中心(桂教科研[2013]20号);广西高校中药药理重点实验室(J1700304)。

摘　　要：目的:研究土牛膝提取物对α-葡萄糖苷酶和二肽基肽酶-4(DPP-4)的抑制作用,探究其降糖作用机制。方法:采用系统溶剂提取法对土牛膝的根、茎、叶进行提取,以α-葡萄糖苷酶和DPP-4为靶点,建立酶活抑制模型,检测土牛膝提取物对酶的抑制作用。结果:土牛膝不同提取物对α-葡萄糖苷酶均有不同程度的抑制作用,其中叶的乙酸乙酯提取物对α-葡萄糖苷酶显示出强抑制活性,IC50为0.18μg/mL。土牛膝不同提取物苷酶;DPP-4;降对DPP-4也有不同程度的抑制作用,其中叶的二氯甲烷提取物对DPP-4显示出强抑制活性,IC50为44.57μg/mL。结论:土牛膝提取物对这两种酶均有不同程度的抑制作用。Objective:To study the inhibitory effects of Achyranthes aspera L.(AL)onα-glucosidase and dipeptidyl peptidase-4(DPP-4),and explore its mechanism of hypoglycemic action.Methods:The root,stem and leaf of AL were extracted by systematic solvent extraction method.Takingα-glucosidase and DPP-4 as targets,the inhibitory activity of AL extracts onα-glucosidase was evaluated by establishing enzyme inhibition model.Results:The root,stem and leaf extracts of AL showed different degrees of inhibition onα-glucosidase.The IC50 of all the ethyl acetate extracts onα-glucosidase was about 15μg/mL,showing good inhibitory activity.The ethyl acetate extracts of AL showed strong inhibitory activity onα-glucosidase.IC50 was 0.18μg/mL.Different extracts of AL also had different inhibitory effects on DPP-4,and the dichloromethane extract of AL showed strong inhibitory activity on DPP-4 with IC50 of 44.57μg/mL.Conclusion:The extract of AL has different inhibitory effects onα-glucosidase and dipeptidyl peptidase-4.

关 键 词：土牛膝 α-葡萄糖血糖 DPP-4 降血糖作用 

分 类 号：TQ[化学工程]