基于2D-COS分析不同热解温度及分子量生物炭DOM的光谱特征  

Spectral characterization of dissolved organic matter derived from biochar with different pyrolysis temperatures and molecular weights based on 2D-COS analysis

作　　者：肖颖晨 汪王宇 聂明华 晏彩霞 丁明军[1] 陈洁[1] XIAO Yingchen;WANG Wangyu;NIE Minghua;YAN Caixia;DING Mingjun;CHEN Jie(Key Laboratory of Poyang Lake Wetland and Watershed Research,Ministry of Education,Jiangxi Province Key Laboratory of Ecological Intelligent Monitoring and Comprehensive Treatment of Watershed,School of Geography and Environment,Jiangxi Normal University,Nanchang 330022)

机构地区：[1]江西师范大学地理与环境学院,鄱阳湖湿地与流域研究教育部重点实验室,流域生态智能监测与综合治理江西省重点实验室,南昌330022

出　　处：《环境科学学报》2025年第3期336-349,共14页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.42067058,42067034);江西省自然科学基金(No.20242BAB23026,20232ACB213014,20232BAB203083)。

摘　　要：选取喜旱莲子草为生物炭原料,利用多种光谱技术和方法,探究不同热解温度(300~700℃)和分子量(0.3、1、3和10 kDa)生物炭溶解性有机质(BDOM)的理化特征,以期为生物炭的应用和入侵植物资源化利用提供理论依据.通过三维荧光光谱结合平行因子分析法共解析出2个类腐殖质和1个类蛋白质组分,各热解温度BDOM总体均以类腐殖物质含量更高,随热解温度升高类蛋白物质百分比逐渐增加.二维相关图谱(2D-COS)结合同步荧光光谱(SFS)分析结果表明,类富里酸对热解温度变化较敏感,并以类腐殖质组分的变化更早;而结合傅里叶变换红外光谱(FTIR)的分析结果表明,酚类和酰胺类物质随热解温度变化的方向不同且快于其他官能团.移动窗口2D-COS分析结果表明,500℃时荧光物质和官能团的响应最强.当透析袋孔径为6 kDa左右时,各BDOM体系荧光组分的变化最显著.而基于SFS-FTIR的Hetero-2DCOS分析表明,主要官能团与类富里酸均随热解温度发生变化,其中,N−H(酰胺II)的变化快于类富里酸,但方向相反.此外,不同分子量BDOM透析过程中,低热解温度以类络氨酸组分较敏感,而高热解温度以类富里酸组分更敏.In this study,Alternanthera philoxeroides were taken as the biochar feedstocks,and various spectroscopic techniques and methods were employed to investigate the physicochemical properties of BDOM pyrolyzed at different temperatures(300~700℃)and with different molecular weights(0.3,1,3 and 10 kDa).This study aims to provide data for biochar application and the resource utilization of invasive plants.Combined with threedimensional fluorescence spectroscopy and parallel factor analysis,two humic-like components and one protein-like component were identified.The dominant fluorescent component of BDOM at each pyrolysis temperature was the humic-like substance.The proportion of protein-like substances increased gradually with increasing pyrolysis temperature.The two-dimensional correlation spectroscopy(2D-COS)combined with synchronous fluorescence spectroscopy analysis results revealed that the fulvic-like component was more sensitive to increasing pyrolysis temperature and the humiclike substance changed preferentially.2D-COS combined with Fourier transform infrared spectroscopy(FTIR)indicated that the direction of change in phenolic-OH and amide II(N−H)depended on pyrolysis temperature,differing from and preceding other functional groups.Furthermore,movingwindow 2D-COS analysis suggested that the response of the fluorescence substances and the functional groups was strongest at 500℃.When the pore size of the dialysis bag is aproximately 6 kDa,the fluorescence components of each BDOM system exhibit the most significant changes.Additionally,Hetero-2DCOS analysis based on SFS and FTIR demonstrated that both the main functional groups and fulvic-like substances changed with increased pyrolysis temperature,among which the change in N−H(amide II)occurred preferentially than that of fulvic-like substances,with an opposite direction.In addition,tyrosine-like and fulvic-like substances were more sensitive to increased molecular weight with BDOM pyrolyzed at low and high temperatures during the dialysis process,respect

关 键 词：生物炭溶解性有机质(BDOM) 热解温度 分子量 光谱分析 二维相关图谱分析(2D-COS) 

分 类 号：X131[环境科学与工程—环境科学]