菊苣对高效氯氰菊酯所致小鼠肝脏损伤的保护作用  

Protective Effect of Chicoryon Cypermethrin-induced Liver Injury in Mice

作　　者：高博 崔新刚[1] 宋美鑫 吴明钊 邱艳荣 贾瑞阳 孙雅欣[1] GAO Bo;CUI Xingang;SONG Meixin;WU Mingzhao;QIU YanRong;JIA Ruiyang;SUN Yaxin(Mudanjiang Medical University,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《中国民族民间医药》2025年第5期30-34,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(2021-KYYWF-0481);黑龙江省省属高等学校基本科研业务费科研项目(2021-KYYWF-0549);牡丹江医学院大学生科研立项项目(2023119)。

摘　　要：目的:探究由农药高效氯氰菊酯菊(β-Cypermethrin,β-CYP)诱导建立的小鼠肝脏损伤模型中,菊苣乙醇提取物(CG-Ι)对农药所致肝脏损伤的保护作用。方法:80%乙醇对菊苣粉进行回流提取浸膏以备用;取60只清洁级小鼠随机分为正常对照组、CG-Ι低剂量组、CG-Ι中剂量组、CG-Ι高剂量组、农药组、中药组;正常对照组灌胃生理盐水、CG-Ι低剂量组灌胃CG-Ι(50 mg/kg)+β-CYP(40 mg/kg)、CG-Ι中剂量组灌胃CG-Ι(100 mg/kg)+β-CYP(40 mg/kg)、CG-Ι高剂量组灌胃CG-Ι(200 mg/kg)+β-CYP(40 mg/kg)、农药组灌胃β-CYP(40 mg/kg)、中药组灌胃CG-Ι(100 mg/kg);实验期间观察小鼠毛发、精神状态等基本情况并记录体重;持续4周后,收集小鼠肝脏并称重,对肝组织进行苏木精-伊红(hematoxylin-eosin,HE)染色观察小鼠肝脏组织结构;同时检测小鼠肝组织匀浆中氧化损伤指标谷丙转氨酶(ALT)、天冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、丙二醇(MDA)水平。结果:随着实验的进行农药组与低剂量组小鼠毛发变得混乱,精神状态低迷;除低剂量组和农药组小鼠体重增长缓慢外,其余各组小鼠体重增长正常;低剂量组和农药组小鼠肝脏器系数相较于其余各组出现异常;低剂量组和农药组小鼠ALT、AST表达水平较高,而中、高剂量组和中药组小鼠表达水平较低与正常对照组相近;低剂量组和农药组小鼠SOD活力较低,而中、高剂量组和中药组小鼠SOD活力较高且与正常对照组相近;低剂量组和农药组小鼠MDA表达水平较高,而中、高剂量组和中药组小鼠MDA表达水平较低与正常对照组相差较小。肝脏病理切片结果显示中、高剂量CG-Ι对β-CYP所诱导的小鼠肝脏组织病理学变化有良好的治疗效果。结论:与正常对照组相比较,低剂量CG-Ι对小鼠ALT、AST、SOD、MDA水平的调控无显著作用且体重增长缓慢,脏器系数异常,因而对肝脏的保护作用不明显;而中、高�Objective To investigate the protective effect of chicory ethanol extract(CG-Ι)on pesticide-induced liver injury in a mouse liver injury model induced by the pesticide cypermethrin(β-Cypermethrin,β-CYP).Methods Chicory powder was refluxed with 80%ethanol to extract for later use;Sixty clean-grade mice were randomly divided into normal control group,CG-Ιlow-dose group,CG-Ιmedium-dose group,CG-Ιhigh-dose group,pesticide group and traditional Chinese medicine group;The normal control group was given normal saline by gavage;CG-Ιlow-dose group was given CG-Ι(50 mg/kg)+β-CYP(40 mg/kg);CG-Ιmedium-dose group were given CG-Ι(100 mg/kg)+β-CYP(40 mg/kg);The high-dose CG-Ιgroup was given CG-Ι(200 mg/kg)+β-CYP(40 mg/kg);The pesticide group was givenβ-CYP(40 mg/kg),and the traditional Chinese medicine group was given CG-Ι(100 mg/kg);During the experiment,the basic conditions such as the hair and mental state of the mice were observed,and the body weight was recorded;After 4 weeks of experimentation,the livers of mice were weighed,and the liver tissues were stained with hematoxylin-eosin(HE)to observe the structure of mouse liver tissues,and the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),superoxide dismutase(SOD)and propylene glycol(MDA),which are indicators of oxidative damage in the homogenates of mouse liver tissues,were detected.Results Mice in the pesticide group and the low-dose group had chaotic hair and low mental state in the later stage;Except for the low-dose group and the pesticide group,the weight gain of the mice in the other groups was normal;Compared with the other groups,the liver organ coefficient of mice in the low-dose group and the pesticide group was abnormal;The expression levels of ALT and AST in the low-dose group and pesticide group were higher,and the levels of ALT and AST in the medium-dose and high-dose groups and the traditional Chinese medicine group were lower than those in the normal control group;The SOD activity of mice in the low-dose group and th

关 键 词：菊苣 高效氯氰菊酯 肝脏损伤 

分 类 号：R285.5[医药卫生—中药学]