CLDN6、TRIM59、CMTM6在鼻咽癌组织中的表达及临床意义  

Expression and clinical significance of CLDN6,TRIM59 and CMTM6 in nasopharyngeal carcinoma

作　　者：王丽丽 王旭燕 张培[2] 韩明明 张静 赵明欣 WANG Lili;WANG Xuyan;ZHANG Pei;HAN Mingming;ZHANG Jing;ZHAO Mingxin(Department of Blood Transfusion,Baoding Second Central Hospital,Baoding 072750,China;Department of Otolaryngology,82nd Army Hospital of the Chinese People's Liberation Army;Department of Otolaryngology,the Fifth Hospital of Baoding;Department of Otolaryngology,Dingzhou People's Hospital)

机构地区：[1]保定市第二中心医院输血科,072750 [2]中国人民解放军陆军第八十二集团军医院耳鼻喉科 [3]保定市第五医院耳鼻喉科 [4]定州市人民医院耳鼻喉科

出　　处：《天津医药》2025年第3期272-276,共5页Tianjin Medical Journal

基　　金：保定市科技计划项目(2141ZF163)。

摘　　要：目的探究紧密连接蛋白6(CLDN6)、三结构域蛋白59(TRIM59)、趋化素样因子超家族6(CMTM6)在鼻咽癌(NPC)组织中的表达及临床意义。方法选取135例NPC患者为研究对象,收集所有患者的癌组织(观察组)及癌旁组织(对照组)。所有患者均进行3年随访。根据随访结果将93例生存患者作为生存组,42例死亡患者作为死亡组。采用荧光定量PCR法测定组织CLDN6、TRIM59、CMTM6 mRNA表达;多因素Cox回归分析NPC患者发生死亡的影响因素;Kaplan-Meier法分析NPC患者组织中CLDN6、TRIM59、CMTM6表达与患者预后的关系。结果与对照组相比,观察组组织CLDN6、TRIM59、CMTM6 mRNA表达均升高(P<0.05)。生存组与死亡组组患者的年龄、性别、体质量指数、TNM分期、骨转移、吸烟史、饮酒史、高血压史相比差异无统计学意义。与生存组相比,死亡组患者的NPC家族史的占比以及癌组织CLDN6、TRIM59、CMTM6 mRNA表达均升高(P<0.05);多因素Cox回归分析显示癌组织CLDN6、TRIM59、CMTM6水平升高为NPC患者发生死亡的危险因素(P<0.05);根据癌组织中CLDN6、TRIM59、CMTM6 mRNA表达的平均值将患者划分为低表达组和高表达组,癌组织CLDN6、TRIM59、CMTM6 mRNA高表达组3年生存率明显低于低表达组(P<0.05)。结论NPC组织中CLDN6、TRIM59、CMTM6 mRNA表达均明显提高,其水平升高为NPC患者发生死亡的危险因素,三者表达与患者预后相关。Objective To investigate the expression and clinical significance of Claudin-6(CLDN6),tripartite motifcontaining protein 59(TRIM59)and chemokine-like factor-like MARVEL transmembrane domain containing member 6(CMTM6)in nasopharyngeal carcinoma(NPC)tissue.Methods A total of 135 NPC patients were selected as the study objects,and cancer tissue(observation group)and para-cancer tissue(control group)of all patients were collected.All patients were followed up for 3 years.According to the follow-up results,93 surviving patients were included in the survival group and 42 dead patients were included in the death group.The mRNA expressions of CLDN6,TRIM59 and CMTM6 were determined by fluorescence quantitative PCR.Multivariate Cox regression was used to analyze the influencing factors of death in NPC patients.Kaplan-Meier method was used to analyze the relationship between the expression levels of CLDN6,TRIM59,CMTM6 and the prognosis of NPC patients.Results Compared with the control group,mRNA expressions of CLDN6,TRIM59 and CMTM6 were increased in the observation group(P<0.05).There were no significant differences in age,sex,body mass index,TNM stage,bone metastasis,smoking history,drinking history and hypertension history between the survival group and the death group.Compared with the survival group,the proportion of NPC family history and the mRNA expression of CLDN6,TRIM59 and CMTM6 in cancer tissue were increased in the death group(P<0.05).Multivariate Cox regression analysis showed that the increased levels of CLDN6,TRIM59 and CMTM6 in cancer tissue were influential factors for death of NPC patients(P<0.05).According to the mean expression levels of CLDN6,TRIM59 and CMTM6 mRNA in cancer tissue,patients were divided into the low expression group and the high expression group.The 3-year survival rate of the high expression group was significantly lower than that of the low expression group(P<0.05).Conclusion The mRNA expressions of CLDN6,TRIM59 and CMTM6 in NPC tissue are significantly increased,which is a risk facto

关 键 词：鼻咽癌 紧密连接蛋白6 三结构域蛋白59 趋化素样因子超家族6 

分 类 号：R739.63[医药卫生—肿瘤]