Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb.leaves on the Caco-2 intestinal cell  

作　　者：Mingcong Fan Weijia Lian Wenjun Yao Tingting Li Jiajia Zhao Qiang Li Yanming Guan Yan Li Haifeng Qian Zhiming Rao Li Wang 

机构地区：[1]School of Food Science and Technology,State Key Laboratory of Food Science and Technology,National Engineering Research Center for Functional Food,Jiangnan University,Wuxi 214122,China [2]Department of Food Science and Engineering,College of Light Industry and Food Engineering,Nanjing Forestry University,Nanjing 210037,China [3]College of Cooking Science and Technology,Jiangsu College of Tourism,Yangzhou 225000,China [4]China National Institute of Standardization,Beijing 100089,China [5]China National Research Institute of Food and Fermentation Industries Co.,Ltd.,Beijing 100015,China [6]School of Biotechnology,Jiangnan University,Wuxi 214122,China

出　　处：《Food Science and Human Wellness》2025年第3期958-967,共10页食品科学与人类健康(英文)

基　　金：supported by the China Postdoctoral Science Foundation(2021M701462);the National Natural Science Foundation of China(32072254);the Postdoctoral Research Funding Program of Jiangsu Province(2021K097A);the Fundamental Research Funds for the Central Universities(JUSRP121106);the“Qing Lan Project”of Jiangsu Province。

摘　　要：The dark blue pigment(DBP)is a health ingredient from Vaccinium bracteatum Thunb.leaves used as a functional food supplement.However,the details of transepithelial absorption on the intestinal epithelial cells are barely understood.This study aimed to clarify the absorption properties of DBP in the Caco-2 cell monolayer model and evaluate the effect on the endo-metabolism and barrier function of Caco-2 cells.The results showed that the DBP did not show the dose-dependent toxic effect to Caco-2 cells between 0.25 and 1.5 mg/mL,which did not cause disorder in the normal cell metabolism and absorption activity.The Caco-2 cell monolayer model could absorb DBP by passive and active transport,and the absorptive pattern was dosedependent when the concentration was more than 0.25 mg/mL.During DBP absorption,an increase in m RNA and protein expressions of glucose transporters demonstrated that the glucose transporters were the potential transporter of DBP.But the glucose transport amounts were significantly lowered after 30 min of DBP treatment,indicating that DBP owned the inhibitory effect on glucose transportation.Furthermore,DBP also owned protective effects on the barrier function of intestinal epithelial cells.

关 键 词：Vaccinium bracteatum Thunb.leaf Dark blue pigment Intestinal epithelial cell Transepithelial absorption Epithelial cell barrier 

分 类 号：R73[医药卫生—肿瘤]