Construction of a standardized analysis method for identifying meat quality-related genes in Ordos fine-wool sheep based on transcriptome sequencing data  

作　　者：Bilige Li Xiawei Aoqier Liu Yang Jia Xiangchun Yao Duo Niu Lin 毕力格;李夏伟;敖其尔;刘洋;贾向春;要铎;牛琳(Inner Mongolia Institute of Quality and Standardization,Inner Mongolia Administration for Market Regulation;Standard Verification Laboratory,Inner Mongolia Institute of Quality and Standardization,Inner Mongolia Administration for Market Regulation)

机构地区：[1]Inner Mongolia Institute of Quality and Standardization,Inner Mongolia Administration for Market Regulation [2]Standard Verification Laboratory,Inner Mongolia Institute of Quality and Standardization,Inner Mongolia Administration for Market Regulation

出　　处：《China Standardization》2025年第2期60-64,共5页中国标准化(英文版)

基　　金：funded by the 2023 Inner Mongolia Public Institution High-Level Talent Introduction Scientific Research Support Project,and the Ordos Municipal Science and Technology Major Special Project(Grant No.2022EEDSKJZDZX021).

摘　　要：In this paper,a standardized analysis method is established for identifying meat quality-related genes in Ordos finewool sheep using transcriptome sequencing data.A meticulously standardized approach is utilized to investigate the genetic determinants of meat quality in Ordos fine-wool sheep through transcriptome sequencing analysis.Muscle samples from the longissimus dorsi of one-year-old sheep are collected under controlled conditions,and key texture properties—hardness,elasticity,and chewiness—are measured to categorize samples into high-and low-textural-value groups.Genes significantly associated with meat quality traits are identified through standardized RNA extraction,high-throughput sequencing,and differential gene expression analysis.Functional enrichment analysis reveals their involvement in biological processes such as extracellular matrix organization and metabolic pathways.The findings underscore the pivotal role of standardization in meat quality research,laying a solid scientific foundation for future research on meat quality improvement and molecular breeding.

关 键 词：Ordos fine-wool sheep meat quality characteristics transcriptome sequencing GENES functional enrichment analysis 

分 类 号：R73[医药卫生—肿瘤]