低浓度阿托品对形觉剥夺性近视豚鼠视网膜多巴胺的影响研究  

Study on the Impact of Low-Concentration Atropine on Retinal Dopamine in Guinea Pigs with Form-Deprivation Myopia

作　　者：李洁[1,2] 王英[1] 袁俊杰 杨宇[2] Li Jie;Wang Ying;Yuan Junjie;Yang Yu(College of Optometry,North Sichuan Medical College,Nanchong 637000,China;Department of Ophthalmology,Nanchong Central Hospital Affiliated to North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院眼视光学院,南充637000 [2]川北医学院附属南充市中心医院眼科,南充637000

出　　处：《成都医学院学报》2025年第2期197-201,共5页Journal of Chengdu Medical College

基　　金：四川省卫生健康委员会科研课题(No:20PJ300)。

摘　　要：目的 探究0.01%和0.05%浓度阿托品在豚鼠近视发生发展过程中的防控差异,以及对视网膜合成多巴胺(DA)含量的影响。方法 选取24只豚鼠,按照随机数字表法取6只豚鼠作为空白对照组,其余豚鼠进行形觉剥夺性近视(FDM)模型造模,按剂量的差异分为模型对照组、0.01%阿托品组、0.05%阿托品组,每组6只。0.01%和0.05%阿托品组使用胰岛素注射针分别将50μL含不同浓度阿托品的聚乙烯醇滴眼液滴入相应的造模眼,模型对照组滴入等量聚乙烯醇滴眼液,空白对照组不处理。造模前后,采用带状光检影镜、眼A超分别检测4组豚鼠左眼屈光度、眼轴长度;造模结束后,采用免疫荧光染色法检测视网膜中酪氨酸羟化酶(TH)阳性表达面积占比。结果 经给予不同浓度阿托品4周后,0.01%和0.05%阿托品组豚鼠造模眼的眼轴延长情况和屈光度下降情况均得到一定程度的改善(P<0.05)。0.05%阿托品组在眼轴延长程度和屈光度降低幅度方面的改善效果优于0.01%阿托品组(P<0.05)。与模型对照组相比,0.01%阿托品组和0.05%阿托品组豚鼠视网膜TH阳性表达面积占比均显著增高(P<0.05),0.05%阿托品组豚鼠视网膜TH阳性表达面积占比高于0.01%阿托品组(P<0.05)。结论 低浓度阿托品滴眼液可有效延缓近视进展,其中0.05%阿托品较0.01%阿托品具有更优的防控效果,其作用机制可能是通过上调视网膜TH表达,促进DA合成增加,从而抑制眼轴增长,减缓近视发生与发展。Objective To study the prevention and control difference between 0.01%and 0.05%atropine concentration in myopia development and their effect on retinal dopamine(DA)content.Methods A total of 24 guinea pigs were selected,among which 6 guinea pigs were selected as the blank control group according to random number table method.The rest guinea pigs underwent form-deprivation myopia(FDM)modeling and were divided into a model control group,a 0.01%atropine group and a 0.05%atropine group according to the dose difference,with 6 guinea pigs in each group.In the 0.01%and 0.05%atropine groups,50μL of polyvinyl alcohol(PVA)eye drops containing the corresponding concentration of atropine were dripped into the corresponding modeling eyes with insulin injection needle,while an equal amount of PVA eye drops was dripped into the corresponding modeling eyes in the model control group.And no treatment was performed in the blank control group.Before and after modeling,the refraction and axial length of the left eye of all guinea pigs included were detected by streak retinoscope and A-mode ultrasound,respectively.After modeling,the percentage of tyrosine hydroxylase(TH)-positive area in the retina was detected by immunofluorescence staining.Results After 4 weeks of atropine administration,the axial elongation and refraction decline of guinea pig model eyes were improved to a certain extent,and the differences were statistically significant(P<0.05).Moreover,the degree of axial elongation and the degree of refraction reduction in the 0.05%atropine group were greater than those in the 0.01%atropine group,and the differences were statistically significant(P<0.05).At the end of modelling,the percentage of TH-positive area in the retinas of guinea pigs in the 0.01%and 0.05%atropine groups was significantly higher than that in the model control group(P<0.05),and the indicator in the 0.05%atropine group was higher than that in the 0.01%atropine group(P<0.05).Conclusion Low-concentration atropine eye drops can effectively slow down the prog

关 键 词：阿托品 近视防控 多巴胺 眼轴长度 屈光度 

分 类 号：R778.1[医药卫生—眼科]