电针刺激上调大脑中动脉栓塞大鼠海马CA1区Nrf2、GPX4的表达  

Electroacupuncture stimulation up-regulates the expression of Nrf2 and GPX4 in the hippocampal CA1 region of rats with middle cerebral artery occlusion

作　　者：吴琪 金毅豪 刘星辰 吴锋 缪化春 李怀斌 WU Qi;JIN Yihao;LIU Xingchen;WU Feng;MIAO Huachun;LI Huaibin(Graduate Student of Grade 2023,Wannan Medical College,Wuhu 241002,China;Department of Human Anatomy,School of Basic Medicine,Wannan Medical College)

机构地区：[1]皖南医学院,安徽芜湖241002 [2]皖南医学院基础医学院人体解剖学教研室

出　　处：《沈阳医学院学报》2025年第2期149-154,186,共7页Journal of Shenyang Medical College

基　　金：安徽省高等学校省级质量工程项目(No.2019jxtd072);安徽省高等学校科研计划重点项目(No.2024AH051927);皖南医学院大学生科研基金研究生类项目(No.WK2023XS56)。

摘　　要：目的:观察电针刺激对大脑中动脉栓塞大鼠海马CA1区Nrf2、GPX4蛋白表达的影响及神经保护作用机制。方法:将48只大鼠按照随机数字表法分为正常组、假手术对照组、模型组和电针组,对模型组、电针组大鼠采用改良后线栓法建立右侧大脑中动脉栓塞模型。电针组在造模后第1天在大鼠“百会”穴(GV20)、左侧“内关”穴(PC6)处予以电针刺激,持续14 d,每天30 min,正常组不做处理,假手术对照组仅分离血管,不插入线栓。采用Zea Longa评分表评估大鼠神经功能受损情况,以Nissl染色法观察其海马CA1区上Nissl阳性细胞的结构与排列情况,采用Western blot和免疫组化染色测定该区域Nrf2、GPX4蛋白表达水平。结果:与正常组比较,假手术对照组大鼠神经功能无缺损,Nissl阳性细胞表达丰富,Nrf2和GPX4蛋白表达及对应阳性细胞数的差异均无统计学意义。与假手术对照组比较,模型组大鼠Zea Longa评分升高,海马CA1区Nissl阳性细胞数减少,Nrf2及GPX4蛋白表达水平、对应阳性细胞数均减少(P<0.05)。与模型组比较,电针组大鼠Zea Longa评分降低,海马CA1区Nissl阳性细胞数增多,Nrf2和GPX4蛋白表达水平及相应阳性细胞数量均增加(P<0.05)。结论:电针刺激可保护大脑中动脉栓塞大鼠海马CA1区神经损伤,其潜在机制可能与上调Nrf2、GPX4的表达有关。Objective:To investigate the effects of electroacupuncture stimulation on the expression of Nrf2 and GPX4 proteins in the hippocampal CA1 region of rats with middle cerebral artery occlusion(MCAO)and its neuroprotective mechanism.Methods:Forty-eight rats were divided into the normal group(Normal),the sham operation control group(Sham),the model group(MCAO)and the electroacupuncture group(EA)according to the random number table method.The right middle cerebral artery occlusion model was established in the MCAO and EA groups by the modified thread embolization method.Starting on day 1 post-modeling,the EA group received electroacupuncture stimulation at the"Baihui"point(GV20)and left"Neiguan"point(PC6)for 30 min daily over 14 d.The Normal group received no treatment,and the Sham group only underwent vascular isolation without inserting the suture.Neurological function of rats in each group were scored before and after modeling and before and after electroacupuncture stimulation using the Zea Longa scoring scale.The structure and arrangement of Nissl-positive cells in the hippocampal CA1 region were observed by Nissl staining.Western blot and immunohistochemical staining were used to determine the expression levels of Nrf2 and GPX4 proteins in the hippocampal CA1 region.Results:Compared with the Normal group,the Sham group showed no neurological deficits,abundant Nissl-positive cells,and no statistically significant differences in Nrf2 and GPX4 protein expression or positive cell counts.Compared with the Sham group,the MCAO group exhibited elevated Zea Longa score,reduced Nissl-positive cell counts,and decreased Nrf2 and GPX4 protein expression and positive cell counts(P<0.05).In contrast,the EA group demonstrated lower Zea Longa scores,increased Nissl-positive cell counts,and elevated Nrf2 and GPX4 protein expression and positive cell counts compared to the MCAO group(P<0.05).Conclusion:Electroacupuncture stimulation may protect against neuronal damage in the hippocampal CA1 region of MCAO rats,potentially through

关 键 词：电针 缺血性卒中 海马CA1区 NRF2 GPX4 

分 类 号：R743.3[医药卫生—神经病学与精神病学]