石仙桃内生真菌的分离及抑菌活性菌株的筛选与鉴定  

Isolation of endophytic fungi from Pholidota chinensis Lindl.and screening and identification of antibacterial strains

作　　者：朱华[1] 吴文源 张淼[1] 陈龙[1] 杨丽容 梁仁涛 黄诗萍 吴焕 施晓光 ZHU Hua;WU Wen-yuan;ZHANG Miao;CHEN Long;YANG Li-rong;LIANG Ren-tao;HUANG Shi-ping;WU Huan;SHI Xiao-guang(Guangxi University of Chinese Medicine/Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine/Guangxi Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine/Guangxi Zhuang Autonomous Region Ethnic Medicine Resources and Application Engineering Research Center,Nanning 530200,China;Guangxi Institute for Drug Control,Nanning 530021,China)

机构地区：[1]广西中医药大学/广西壮瑶药重点实验室/广西壮瑶药协同创新中心/广西壮族自治区民族药资源与应用工程研究中心,南宁530200 [2]广西壮族自治区药品检验研究院,南宁530021

出　　处：《湖北农业科学》2025年第2期112-121,共10页Hubei Agricultural Sciences

基　　金：2024年度广西高校中青年教师科研基础能力提升项目(2024KY0310);广西科技基地和人才专项(桂科AD21238031);广西药监局药品安全科研计划项目(桂药监科直属[2023]035号);广西中医药重点学科壮药学项目(GZXK-Z-20-64);广西一流学科中药学(民族药学)项目(桂教科研[2018]12号);广西科协“提质增效”项目。

摘　　要：从传统药用植物石仙桃(Pholidota chinensis Lindl.)中,分离对致病细菌具有抑菌作用的内生真菌菌株,研究菌株的抗菌活性。采用组织块法分离石仙桃内生真菌,采用纸片法进行抑菌试验,筛选出有抑菌活性的菌株;根据其形态特征,筛选有抑菌活性的菌株,并进行DNA提取和鉴定。结果表明,从石仙桃根、根茎、叶和假鳞茎中分离得到49株内生真菌,其中,广西河池市石仙桃分离出37株内生真菌,福建省漳州市石仙桃分离出12株内生真菌,从叶部组织中分离到的广西河池市石仙桃内生真菌HCY-5、HCY-9、HCY-10菌株和福建省漳州市石仙桃内生真菌FJY-2、FJY-5、FJY-6、FJY-7菌株对金黄色葡萄球菌(Staphylococcus aureus)表现出较好的抑菌活性,结合形态特征,将测得内生真菌菌株基因序列导入GenBank,经过比对,鉴定广西河池市石仙桃内生真菌HCY-5、HCY-9、HCY-10菌株和福建省漳州市石仙桃内生真菌FJY-2、FJY-5、FJY-6、FJY-7菌株为Phyllosticta(叶点霉属),推测为Phyllosticta capitalensis(意大利果壳叶点霉)。广西河池市石仙桃内生真菌HCY-5、HCY-9、HCY-10和福建省漳州市石仙桃内生真菌FJY-2、FJY-5、FJY-6、FJY-7菌株具有较好的抑菌活性。Endophytic fungi strains with antifungal activity against pathogenic bacterial were isolated from the traditional medicinal plant Pholidota chinensis,and the antifungal activity of the strains was studied.Endophytic fungi of Pholidota chinensis were isolated by tissue block method and fungal inhibition test was carried out by paper method,and the strains with antibacterial activity were screened out.According to their morphological characteristics,the active strains with antibacterial activity were screened for DNA ex⁃traction and identification.The results showed that 49 endophytic fungi were isolated from the roots,rhizomes,leaves and pseudobulb of Pholidota chinensis,including 37 endophytic fungi from Hechi City,Guangxi and 12 endophytic fungi from Zhangzhou City,Fujian Province.Endophytic fungi HCY-5,HCY-9,HCY-10 isolated from the leaf tissues of Pholidota chinensis in Hechi City,Guangxi Province,and endophytic fungi FJY-2,FJY-5,FJY-6,FJY-7 isolated from Pholidota chinensis in Zhangzhou City,Fujian Prov⁃ince,showed good antibacterial activity against Staphylococcus aureus.Combined with morphological characteristics,the gene se⁃quences of endophytic fungi strains were introduced into GenBank,and after comparison,the endophytic fungi HCY-5,HCY-9,HCY-10 in Hechi City,Guangxi and FJY-2,FJY-5,FJY-6,FJY-7 in Zhangzhou City,Fujian Province were identified as Pholiost⁃icta,which was supposed to be Pholiosticta capitalensis.Endophytic fungi HCY-5,HCY-9,HCY-10 from Hechi City,Guangxi and endophytic fungi FJY-2,FJY-5,FJY-6 and FJY-7 from Zhangzhou City,Fujian Province had good antibacterial activity.

关 键 词：石仙桃(Pholidota chinensis Lindl.) 内生真菌 抑菌活性 筛选 鉴定 

分 类 号：R284.2[医药卫生—中药学] S567.239[医药卫生—中医学]