荒漠肉苁蓉总低聚糖提取工艺优化及生物活性分析  

Optimization of extraction process and biological activity analysis of total oligosaccharides from Cistanche deserticola

作　　者：田占萍 王宇 吉舒仪 谭峻杰 姚军[1] 沈静 TIAN Zhan-ping;WANG Yu;JI Shu-yi;TAN Jun-jie;YAO Jun;SHEN Jing(School of Pharmacy,Xinjiang Medical University,Urumqi 830017,China;Department of Pharmacy,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Department of Pharmacy,The Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830017 [2]新疆医科大学第一附属医院药学部,乌鲁木齐830054 [3]新疆医科大学第五附属医院药学部,乌鲁木齐830011

出　　处：《湖北农业科学》2025年第2期156-161,178,共7页Hubei Agricultural Sciences

基　　金：新疆维吾尔自治区重大科技专项项目(2022A03007-2);新疆维吾尔自治区自然科学基金项目(2022D01C327)。

摘　　要：采用超声辅助法提取荒漠肉苁蓉(Cistanche deserticola)总低聚糖,通过单因素和正交试验优化提取条件;运用红外光谱初步解析荒漠肉苁蓉总低聚糖的结构特征;通过测定α-葡萄糖苷酶的抑制能力、DPPH、·OH及ABTS自由基的清除率研究荒漠肉苁蓉总低聚糖的体外降糖能力和体外抗氧化活性。结果表明,荒漠肉苁蓉总低聚糖的最佳提取工艺条件为料液比1∶50,乙醇浓度40%,超声时间32 min,在该条件下总低聚糖得率达17.32%;红外光谱图表明有明显的总低聚糖特征吸收,且为β-型吡喃糖类;生物活性结果显示对α-葡萄糖苷酶的抑制率达63.94%,对DPPH、·OH及ABTS自由基清除率分别达85.63%、60.91%、82.90%。该方法有助于提高荒漠肉苁蓉总低聚糖得率,且其具有较强的体外抗氧化和降糖能力,可为相关研究提供参考。The total oligosaccharides from Cistanche deserticola were extracted by ultrasonic-assisted method,and the extraction con⁃dition was optimized by single factor and orthogonal test.The structural characteristics of total oligosaccharides of Cistanche deserticola were preliminarily analyzed by infrared spectroscopy.The in vitro hypoglycemic ability and antioxidant activity of total oligosaccharides from Cistanche deserticola were studied by measuring the inhibition ability ofα-glucosidase,and the scavenging rate of DPPH,·OH and ABTS free radicals.The results showed that the optimum extraction condition of total oligosaccharides from Cistanche deserticola was the solid-liquid ratio of 1∶50,ethanol concentration of 40%and ultrasonic time of 32 min.Under this condition,the yield of total oligosaccharides was 17.32%.The infrared spectroscopy showed that there was obvious characteristic absorption of total oligosaccha⁃rides,and it wasβ-pyranose.The results of biological activity showed that the inhibition rate ofα-glucosidase was 63.94%,and the scavenging rates of DPPH,·OH and ABTS free radicals were 85.63%,60.91%and 82.90%,respectively.This method was helpful to improve the yield of total oligosaccharides from Cistanche deserticola,and these total oligosaccharides had strong antioxidant and hypo⁃glycemic ability in vitro,which could provide reference for related research.

关 键 词：荒漠肉苁蓉(Cistanche deserticola) 总低聚糖 超声辅助法提取 抗氧化 降血糖 

分 类 号：R284.2[医药卫生—中药学]