4种马铃薯内源激素提取方法优化及其在块茎解除休眠过程中的含量分析  

Optimization of extraction methods for four endogenous hormones in potatoes and analysis of their content during the process of releasing dormancy in tubers

作　　者：赵喜娟 张帆[2] 刘圣宣 覃骏 陈惠兰[2] 林原 罗红兵[1] 刘易[3] 宋波涛[2] 胡新喜[1] 王恩爽 ZHAO Xi-Juan;ZHANG Fan;LIU Sheng-Xuan;QIN Jun;CHEN Hui-Lan;LIN Yuan;LUO Hong-Bing;LIU Yi;SONG Bo-Tao;HU Xin-Xi;WANG En-Shuang(Hunan Agricultural University/Key Laboratory for Vegetable Biology of Hunan Province/Yuelu Mountain Laboratory,Changsha 410128,Hunan,China;College of Horticulture&Forestry Sciences,Huazhong Agricultural University/Key Laboratory of Potato Biology and Biotechnology,Ministry of Agriculture and Rural Affairs/National Key Laboratory of Germplasm Innovation and Utilization of Horticulture Crops,Wuhan 430070,Hubei,China;Comprehensive Test Site of Xinjiang Academy of Agricultural Sciences,Urumqi 830013,Xinjiang,China Abstract:Plant hormones are essential regulators of growth and development.However,the lack of a unified and efficient method for detecting endogenous hormones in potato has hindered in-depth research.In this study,ultra-high performance liquid chromatography(UPLC)was combined with single-factor and orthogonal experimental designs to optimize the extraction meth)

机构地区：[1]湖南农业大学/湖南省蔬菜生物学重点实验室/岳麓山实验室,湖南长沙410128 [2]华中农业大学园艺林学学院/农业农村部马铃薯生物学与生物技术重点实验室/果蔬园艺作物种质创新与利用全国重点实验室,湖北武汉430070 [3]新疆农业科学院综合试验场,新疆乌鲁木齐830013

出　　处：《作物学报》2025年第4期1050-1060,共11页Acta Agronomica Sinica

基　　金：新疆维吾尔自治区智力援疆创新拓展人才计划“小组团”援疆团队-“马铃薯生物技术与早熟育种团队”项目;国家重点研发计划项目(2023YFD1201505,2022YFD1100201)资助。

摘　　要：植物激素是生长发育的重要调节物质,但马铃薯内源激素还没有统一、高效的检测方法,难以满足深入研究的需求。本研究利用超高效液相色谱技术,设计单因素试验和正交试验,优化了SA、IAA、ABA和JA这4种植物激素的提取方法,其重复性和稳定性的相对标准偏差均小于10%,提取效率显著高于其他提取方法。利用该方法,对生长30 d的马铃薯植株的根、茎、叶、匍匐茎和顶芽的激素含量进行测定发现,顶芽和叶片中SA含量最高,顶芽和匍匐茎中IAA含量最高,叶片中ABA含量最高,根和匍匐茎中JA含量偏高。对马铃薯栽培种华薯1号和中薯5号的块茎分别在4℃、22℃和28℃条件下储存,并检测了其休眠打破过程中激素含量。结果表明,在整个休眠期,IAA含量整体呈上升趋势,SA含量呈先上升后下降而后快速上升的趋势,ABA含量整体呈下降趋势。本研究优化的提取方法提取效率高、灵敏度高、稳定性和重复性好,适用于马铃薯4种内源激素的检测分析,研究数据为马铃薯不同组织和块茎休眠过程中激素合成及作用机制提供理论参考。Plant hormones are essential regulators of growth and development.However,the lack of a unified and efficient method for detecting endogenous hormones in potato has hindered in-depth research.In this study,ultra-high performance liquid chromatography(UPLC)was combined with single-factor and orthogonal experimental designs to optimize the extraction meth ods for four phytohormones:salicylic acid(SA),indole-3-acetic acid(IAA),abscisic acid(ABA),and jasmonic acid(JA).The optimized method demonstrated excellent repeatability and stability,with relative standard deviations(RSDs)below 10%,and significantly improved extraction efficiency compared to conventional methods.Using this optimized method,the hormone contents in the roots,stems,leaves,apical buds,and stolons of potato plants at 30 days of growth were quantified.The results revealed that endogenous SA levels were highest in the apical buds and leaves,IAA was most abundant in the shoot apex and stolons,ABA was concentrated in the leaves,and JA levels were higher in the roots and stolons.Additionally,the tubers of potato cultivars Huashu 1 and Zhongshu 5 were stored at 4°C,22℃,and 28℃,and hormone levels during dormancy breaking were analyzed.The findings showed a general upward trend in IAA levels throughout storage,a fluctuating pattern in SA levels(initial increase,subsequent decline,and rapid rise),and a consistent decrease in ABA levels.The optimized extraction method developed in this study offers high efficiency,sensitivity,stability,and repeatability,making it suitable for the detection and analysis of these four endogenous hormones in potato.This research provides valuable insights into hormone synthesis and their regulatory mechanisms during dormancy and across various tissues in potato.

关 键 词：马铃薯 激素 提取方法 超高效液相色谱 休眠 

分 类 号：S53[农业科学—作物学]