水稻粉质胚乳突变体we2的表型分析与基因定位  

Phenotypic analysis and gene mapping of a floury endosperm mutant we2 in rice

作　　者：朱建平 李文奇[1,2,3] 许扬 王芳权 李霞[1,2,3] 蒋彦婕 范方军[1,2,3] 陶亚军 陈智慧[1,2,3] 吴莹莹 杨杰[1,2,3] ZHU Jian-Ping;LI Wen-Qi;XU Yang;WANG Fang-Quan;LI Xia;JIANG Yan-Jie;FANFang-Jun;TAO Ya-Jun;CHEN Zhi-Hui;WU Ying-Ying;YANG Jie(Institute of Food Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River(Nanjing),Ministry of Agricultural and Rural Affairs/Nanjing Branch of Chinese National Center for Rice Improvement,Nanjing 210014,Jiangsu,China;Zhongshan Biological Breeding Laboratory,Nanjing 210014,Jiangsu,China;Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,Jiangsu,China)

机构地区：[1]江苏省农业科学院粮食作物研究所/农业农村部淮河下游种质创制重点实验室(南京)/国家水稻改良中心南京分中心,江苏南京210014 [2]生物育种钟山实验室,江苏南京210014 [3]扬州大学江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009

出　　处：《作物学报》2025年第4期1110-1117,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32201861);江苏省重点研发计划项目(BE2023362);江苏省种业振兴揭榜挂帅项目(JBGS(2021)008)资助。

摘　　要：从日本晴^(60)Co诱变突变体库中筛选到一份粉质胚乳突变体we2(white endosperm2),对其表型和理化性质进行分析,并利用we2与9311杂交获得的F_(2)群体对目标基因进行精细定位。we2胚乳表现为白色粉质,淀粉颗粒排列疏松且不规则;千粒重、总淀粉和直链淀粉含量显著下降,脂肪含量显著上升。遗传分析表明we2粉质胚乳性状受单个隐性基因控制。利用we2/9311 F_(2)群体进行基因定位,WE2被定位在第6染色体长臂P38和P39之间约244 kb区间内,该区间包含27个开放阅读框(Open reading frames,ORFs)。qRT-PCR结果显示,we2胚乳中淀粉合成相关基因表达显著下调。本研究为WE2的克隆和功能分析奠定了基础。A floury endosperm mutant,we2(white endosperm2),was identified from a^(60)Co-irradiated mutant pool of the rice(Oryza sativa)variety Nipponbare.The phenotype and physicochemical properties of we2 were analyzed,and an F_(2)population derived from a cross between we2 and the indica variety 9311 was used for fine mapping of the target gene.Compared with the wild type,we2 exhibited a white endosperm phenotype with irregularly shaped,loosely packed compound starch granules.The 1000-grain weight,total starch content,and amylose content in we2 were significantly lower than those in the wild type,whereas the lipid content was higher.Genetic analysis revealed that the floury endosperm phenotype of we2 is controlled by a single nuclear recessive gene.For map-based cloning,the we2 mutant was crossed with 9311,and F_(2)individuals were analyzed.The WE2 locus was initially mapped to chromosome 6 and subsequently fine-mapped to a 244 kb genomic region containing 27 predicted open reading frames(ORFs).Quantitative real-time PCR(qRT-PCR)analysis demonstrated that the expression levels of several genes involved in starch biosynthesis were reduced in the we2 mutant.This study provides a foundation for the cloning and functional characterization of WE2,contributing to a deeper understanding of the genetic and molecular mechanisms underlying rice endosperm development.

关 键 词：水稻 胚乳 淀粉 精细定位 

分 类 号：S51[农业科学—作物学]