辽宁省白纹伊蚊对3种拟除虫菊酯类杀虫剂的抗药性及其击倒抗性基因型分布研究  

Resistance of Aedes albopictus to three pyrethroid insecticides and the distribution of their knockdown resistance genotypes in Liaoning Province,China

作　　者：王纯玉[1] 赵春春 邢俊[3] 李华锋 丁俊[1] WANG Chun-yu;ZHAO Chun-chun;XING Jun;LI Hua-feng;DING Jun(Vector Control Institute,Liaoning Center for Disease Control and Prevention,Shenyang,Liaoning 110005,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,WHO Collaborating Centre for Vector Surveillance and Management,Department of Vector Biology and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Dalian Center for Disease Control and Prevention,Dalian,Liaoning 116000,China;Yingkou Center for Disease Control and Prevention,Yingkou,Liaoning 115000,China)

机构地区：[1]辽宁省疾病预防控制中心病媒生物控制所,辽宁沈阳110005 [2]中国疾病预防控制中心传染病预防控制所媒介生物控制室,传染病溯源预警与智能决策全国重点实验室,WHO媒介生物监测与管理合作中心,北京102206 [3]大连市疾病预防控制中心,辽宁大连116000 [4]营口市疾病预防控制中心,辽宁营口115000

出　　处：《中国媒介生物学及控制杂志》2025年第1期22-27,共6页Chinese Journal of Vector Biology and Control

摘　　要：目的了解辽宁省白纹伊蚊对3种拟除虫菊酯类杀虫剂的抗药性水平及其击倒抗性基因型分布特点,为该地区防控白纹伊蚊及规范使用杀虫剂提供科学依据。方法2023年7-8月,在辽宁省大连和营口市白纹伊蚊孳生地采集蚊虫,经形态学方法鉴定为白纹伊蚊后,采用成蚊接触筒法测定其对氯菊酯、溴氰菊酯和高效氯氰菊酯的抗药性。提取单只蚊虫的基因组DNA,PCR扩增电压门控钠离子通道(VGSC)基因部分片段,测序后对白纹伊蚊击倒抗性基因突变情况进行描述性统计分析。结果大连地区白纹伊蚊成蚊对氯菊酯、溴氰菊酯、高效氯氰菊酯为可能抗性种群。营口地区白纹伊蚊成蚊对氯菊酯、溴氰菊酯、高效氯氰菊酯均已产生抗性。共检测2个野外种群110只白纹伊蚊,获得220条基因序列,长度约为400 bp,白纹伊蚊在1016、1532和1534位点均检测到突变。1016位点有2种等位基因,分别为野生型GTA/V(82.73%)和突变型GGA/G(17.27%);有3种基因型,分别为野生型纯合子V/V(68.18%)、野生/突变型杂合子V/G(29.09%)和突变型纯合子G/G(2.73%)。1532位点有2种等位基因,分别为野生型ATC/I(86.82%)和突变型ACC/T(13.18%);有3种基因型,分别为野生型纯合子I/I(76.36%)、野生/突变型杂合子I/T(20.91%)和突变型纯合子T/T(2.73%)。1534位点有4种等位基因,分别为野生型TTC/F(50.46%)、突变型TCC/S(23.64%)、突变型TGC/C(15.45%)和突变型CTC/L(10.45%);有8种基因型,分别为野生型纯合子F/F(24.55%)、野生/突变型杂合子F/S(24.55%)、F/C(11.82%)和F/L(15.45%),突变型纯合子S/S(9.09%)、C/C(7.27%)和L/L(2.73%),突变型杂合子S/C(4.54%)。结论辽宁省大连地区白纹伊蚊成蚊对3种拟除虫菊酯类杀虫剂为可能抗性种群,营口地区白纹伊蚊成蚊为抗性种群,大连和营口市白纹伊蚊均发生击倒抗性基因突变,其中1534位点突变率高且突变情况复杂多样,应持续关注白纹伊蚊抗药性水平,减�Objective To investigate the resistance level of Aedes albopictus to three pyrethroid insecticides and the distribution of their knockdown resistance genotypes in Liaoning Province,China,so as to provide a scientific basis for the prevention and control of Ae.albopictus and the standardized use of insecticides in the region.Methods From July to August 2023,adult mosquitoes and larvae were collected from breeding sites of Ae.albopictus in Dalian and Yingkou in Liaoning Province.After mosquitoes were identified by morphology as Ae.albopictus,their resistance to three pyrethroid insecticides(permethrin,deltamethrin,and beta-cypermethrin)was determined by the adult mosquito WHO tube assay.The genomic DNAs of single mosquito were extracted,and the partial fragment of voltage-gated sodium channel(VGSC)gene was amplified by polymerase chain reaction.Descriptive statistical analysis method was used for the analysis of knockdown resistance genes mutations in Ae.albopictus after sequencing.Results Adult Ae.albopictus mosquitoes in Dalian had have possible resistance to permethrin,deltamethrin,and beta-cypermethrin.Adult Ae.albopictus mosquitoes in Yingkou area had developed resistance to permethrin,deltamethrin,and beta-cypermethrin.A total of 110 Ae.albopictus from two field populations were detected,and 220 gene sequences were obtained,with the length of about 400 bp.Mutations were detected at 1016,1532,and 1534 loci of Ae.albopictus.At the 1016 locus,there were two alleles,namely wild-type GTA/V(82.73%)and mutant GGA/G(17.27%);there were three genotypes,namely wild-type homozygote V/V(68.18%),wild-type/mutant heterozygote V/G(29.09%),and mutant homozygote G/G(2.73%).At the 1532 locus,there were two alleles,namely wild-type ATC/I(86.82%)and mutant ACC/T(13.18%);there were three genotypes,namely wild-type homozygote I/I(76.36%),wild-type/mutant heterozygote I/T(20.91%),and mutant homozygote T/T(2.73%).At the 1534 locus,there were four alleles,namely wild-type TTC/F(50.46%),mutant TCC/S(23.64%),mutant TGC/C(15.45%),and mut

关 键 词：白纹伊蚊 抗药性 击倒抗性 突变 辽宁省 

分 类 号：R384.1[医药卫生—医学寄生虫学]