Histological demonstration of gonad development in the banana shrimp,Fenneropenaeus merguiensis  

作　　者：Jirakanit Chimnual Jirawat Saetan Wilaiwan Chotigeat 

机构地区：[1]Division of Biological Science,Molecular Biotechnology and Bioinformatics Program,Faculty of Science,Prince of Songkla University,Songkhla,90110,Thailand [2]Division of Health and Applied Sciences,Faculty of Science,Prince of Songkla University,Songkhla,90110,Thailand [3]Center for Genomics and Bioinformatics Research,Faculty of Science,Prince of Songkla University,Songkhla,90110,Thailand

出　　处：《Aquaculture and Fisheries》2025年第1期94-104,共11页渔业学报(英文)

基　　金：funding from the Fundamental Fund,Prince of Songkla University(Contact No.SCI6405032b);funding support from the NSRF via the Program Management Unit for Human Resources&Institutional Development,Research and Innovation[grant number B05F630026];Science and Technology Research Partnership for Sustainable Development(SATREPS,Japan Science and Technology Agency(JST,JPMJSA1806)/Japan International Cooperation Agency(JICA);the Faculty of Science Research Fund,Prince of Songkla University[grant number 1-2565-02-001].

摘　　要：The genetic diversity of the banana shrimp Fenneropenaeus merguiensis is at risk because of over-harvesting,which consequently reduces food security.This endangerment is exacerbated because this species is not commonly cultivated by farmers.Overall,these factors necessitate conservation of this shrimp species across its natural habitat.Information on the migration of primordial germ cells(PGCs)to form a gonad is essential for shrimp preservation techniques such as broodstock preparation,sex differentiation,and germ cell transplantation.In this study,histological analysis and in situ hybridization of vasa expression(from embryo to testis development)were used to demonstrate the movement of PGCs.Hematoxylin and Eosin staining and in situ hybridization with the VASA probe revealed that the PGCs migrated retrogradely along the midgut,colonizing the area between the hepatopancreas and heart,a region that becomes the genital ridge in the postlarval stage.External sexual organs appeared at approximately 4 months of age.Through real-time PCR,the expression of the vasa gene was detected early on postlarval day 7,whereas its abundant expression was detected in the ovaries and testes of adult shrimp.This study could help with the identification and monitoring of PGCs or spermatogonia in banana shrimp and facilitate the implementation of other germ cell-relevant techniques in the future.

关 键 词：Fenneropenaeus merguiensis Primordial germ cell SPERMATOGONIA VASA 

分 类 号：Q17[生物学—水生生物学]