放射线照射三阴性乳腺癌细胞后外泌体对Treg细胞的作用  

Role of exosomes on regulatory T cells after radiation irradiation for triple-negative breast cancer cells

作　　者：任金丽 潘驰 倪清涛 Ren Jinli;Pan Chi;Ni Qingtao(Department of acupuncture and physiotherapy,the Affiliated Taizhou People's Hospital of Nanjing Medical University,Taizhou School of Clinical Medicine,Nanjing Medical University,Taizhou 225300,China;Department of General Surgery,the Affiliated Taizhou People's Hospital of Nanjing Medical University,Taizhou School of Clinical Medicine,Nanjing Medical University,Taizhou 225300,China;Department of Oncology,the Affiliated Taizhou People's Hospital of Nanjing Medical University,Taizhou School of Clinical Medicine,Nanjing Medical University,Taizhou 225300,China)

机构地区：[1]南京医科大学附属泰州人民医院针灸理疗科,南京医科大学泰州临床医学院,泰州225300 [2]南京医科大学附属泰州人民医院普外科,南京医科大学泰州临床医学院,泰州225300 [3]南京医科大学附属泰州人民医院肿瘤科,南京医科大学泰州临床医学院,泰州225300

出　　处：《中华放射肿瘤学杂志》2025年第3期289-294,共6页Chinese Journal of Radiation Oncology

基　　金：江苏省双创博士项目(202003205,202003206);南京医科大学泰州临床医学院科研项目-国自然(青年基金)培育专项(TZKY20220105,TZKY20220109)。

摘　　要：目的探讨放射线照射后三阴性乳腺癌(TNBC)细胞释放的外泌体对免疫细胞的影响。方法培养3种TNBC细胞(MDA-MB-231、MDA-MB-453、MDA-MB-468细胞)生长至70%愈合度时,一组给予8 Gy剂量照射,另一组无干预措施。照射后48 h收集细胞培养上清液。随后,将上清液与外周血单个核细胞中的淋巴细胞共同培养,并通过荧光显微镜拍照确认T细胞对外泌体的摄取情况。同时,使用流式细胞术检测细胞中调节性T细胞(Treg细胞)的表达情况。通过t检验比较两组间Treg细胞分化差异(用Treg细胞阳性率表示)。结果透射电子显微镜扫描及纳米粒子分析法分析显示,实验中提取的外囊泡为外泌体。淋巴细胞将外泌体吞噬,吞噬后外泌体主要集中在细胞质。3种三阴性乳腺癌细胞释放的外泌体与淋巴细胞共同培养后,与空白组比较,Treg细胞分化增加(1.07%、0.60%、0.63%比0.30%,P<0.01)。此外,照射后三阴性乳腺癌细胞释放的外泌体进一步增加了Treg细胞的分化(MDA-MB-231组1.07%比1.81%,P<0.01;MDA-MB-453组0.6%比0.93%,P<0.05)。结论TNBC细胞释放的外泌体可以促进Treg细胞的分化,而照射后的TNBC细胞释放的外泌体进一步促进了Treg细胞的分化。ObjectiveTo investigate the effect of exosomes released from triple-negative breast cancer cells(TNBC)on immune cells after radiotherapy.MethodsWhen TNBC(3 types:MDA-MB-231,MDA-MB-453,MDA-MB-468)reached 70%confluence,cells were irradiated with a dose of 8 Gy in one group and no intervention was given in the control group.The supernatants were collected at 48 h after irradiation.Subsequently,these supernatants were co-cultured with lymphocytes in peripheral blood mononuclear cells,and the uptake of exosomes by T cells was confirmed by fluorescence microscopy.Meanwhile,the expression of regulatory T cells(Treg)in the cells was detected using flow cytometry.Differences in Treg cell differentiation between two groups were compared by t-test(expressed as Treg cell positivity rate).ResultsTransmission electron microscopy scanning and nanoparticle analysis showed that the extracellular vesicles extracted in the experiment were exosomes.Lymphocytes phagocytosed the exosomes and the exosomes were mainly concentrated in the cytoplasm after phagocytosis.Following the co-culture of 3 kinds of lymphocytes with exosomes from TNBC,there was an increase in Treg cell differentiation compared to control group(1.07%,0.60%,0.63%vs.0.30%,P<0.01).In addition,exosomes released from TNBC further increased the differentiation of Treg cells after radiotherapy(MDA-MB-231 cells:1.07%vs.1.81%,P<0.01;MDA-MB-453 cells:0.60%vs.0.93%,P<0.05).ConclusionsIn summary,exosomes released from TNBC can promote the differentiation of Treg cells.In addition,radiotherapy-induced exosomes released by TNBC further exacerbate the differentiation of Treg cell.

关 键 词：外泌体 三阴性乳腺癌 放射疗法 T淋巴细胞 调节性 

分 类 号：R73[医药卫生—肿瘤]