固相萃取-超高效液相色谱法测定茶叶中11种合成着色剂  

Determination of 11 synthetic colorants in tea by solid phase extraction-ultra performance liquid chromatography

作　　者：曾豪威 周庆琼 黄铁城 陈羽中 邱佩丽 戚平 ZENG Haowei;ZHOU Qingqiong;HUANG Tiecheng;CHEN Yuzhong;QIU Peili;QI Ping(Guangzhou Institute for Food Inspection,Guangzhou 511400,China)

机构地区：[1]广州市食品检验所,广州511400

出　　处：《分析试验室》2025年第2期247-252,共6页Chinese Journal of Analysis Laboratory

基　　金：国家市场监督管理总局科技计划项目(2020MK129);广州市市场监督管理局科技项目(2023KJ39,2022KJ41)资助。

摘　　要：基于固相萃取-超高效液相色谱法,建立了茶叶中11种合成着色剂的定量分析方法。样品经甲醇水提取,WAX固相萃取柱净化,超高效液相色谱仪测定,外标法定量。结果表明,11种合成着色剂在1~50μg/mL浓度范围内线性关系良好,相关系数均大于0.99,检出限为0.020~0.188 mg/kg,定量限为0.060~0.564 mg/kg。加标回收率在81.0%~108.0%之间,相对标准偏差为0.11%~3.8%。对市售192批次茶叶样品进行检测,7批次样品中检出柠檬黄和日落黄。本方法适用于茶叶中合成着色剂的定量分析。Based on solid-phase extraction and ultra performance liquid chromatography(SPE-UPLC),a method for the quantitative analysis of 11 synthetic colorants in tea was established.The samples were extracted with methanol/water mixed solvents,purified by the Weak Anion Exchange(WAX)solid phase extraction column,determined by UPLC,and quantified by the external standard method.The results showed that the 11 synthetic colorants exhibited good linear relationships in the concentration range of 1-50μg/mL,with correlation coefficients all greater than 0.99.The limits of detection(LODs)ranged from 0.020 to 0.188 mg/kg,and the limits of quantitation(LOQs)were 0.060-0.564 mg/kg.The recovery rates ranged from 81.0%to 108.0%,with the relative standard deviations of 0.11%-3.8%.Tartrazine and Sunset Yellow were detected in 7 batches of commercially available 192 batches of tea samples.This method is suitable for the quantitative analysis of synthetic colorants in tea.

关 键 词：超高效液相色谱 固相萃取 茶叶 合成着色剂 

分 类 号：O657.7[理学—分析化学]