牛磺酸调控BDNF/TrkB信号通路并对小于胎龄大鼠神经元起保护作用  

Taurine Regulates BDNF/TrkB Signaling Pathway and Protects Neurons in Rats with Small Gestational Age

作　　者：芮菲菲 朱荣平 RUI Feifei;ZHU Rongping(Changzhou Maternal and Child Health Care Hospital,Changzhou 213000,Jiangsu,China)

机构地区：[1]常州市妇幼保健院,中国江苏常州213000

出　　处：《生命科学研究》2025年第1期41-48,共8页Life Science Research

基　　金：南京医科大学科技发展基金一般项目(NMUB20220198)。

摘　　要：为了探究牛磺酸(2-aminoethanesulfonic acid,taurine)对小于胎龄(small for gestational age,SGA)大鼠神经元的保护作用和分子机制,利用全程饮食限制法建立SGA大鼠模型,利用Longa、Bederson等评分考察牛磺酸对SGA大鼠神经功能的影响,利用免疫荧光染色和免疫印迹分析考察牛磺酸对SGA大鼠海马体神经炎症和神经损伤的影响,利用实时荧光定量PCR(quantitative real-time PCR,qPCR)和酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)考察牛磺酸对SGA大鼠炎症因子水平的影响,利用TUNEL检测技术考察牛磺酸对SGA大鼠神经元凋亡的影响,利用免疫印迹分析考察牛磺酸对SGA大鼠脑部脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、酪氨酸激酶受体B(tyrosine receptor kinase B,TrkB)和p-TrkB表达的影响。结果显示,牛磺酸抑制了SGA大鼠海马体神经炎症和神经损伤,并抑制了SGA大鼠的神经元凋亡,从而对SGA大鼠神经功能起到改善作用。除此之外,牛磺酸促进了大鼠脑组织BDNF/TrkB信号通路的激活。上述结果表明,牛磺酸促进SGA大鼠中BDNF/TrkB信号通路的激活,并可能经由此通路对SGA大鼠的神经元起保护作用。In order to explore the protective effect and molecular mechanism of 2-aminoethanesulfonic acid(taurine)on neurons of rats born small for gestational age(SGA),the SGA rat model was established with whole-diet restriction method.The effects of taurine on nerve functions in SGA rats were assessed using Longa and Bederson scores,and the effects of taurine on hippocampus neuroinflammation and nerve injury were evaluated by immunofluorescence staining and Western-blot.The levels of inflammatory factors were detected by quantitative real-time PCR(qPCR)and enzyme linked immunosorbent assay(ELISA).The neuron apoptosis and brain-derived neurotrophic factor(BDNF),tyrosine receptor kinase B(TrkB)and p-TrkB expressions were examined by TUNEL assay and Western-blot,respectively.The results showed that taurine inhibited neuroinflammation and nerve damage in the hippocampus,and prevented neuronal apoptosis,thereby improving nerve functions in SGA rats.In addition,taurine promoted the activation of BDNF/TrkB signaling pathway in rat brain tissue.Therefore,taurine may protect neurons in SGA rats through promoting the activation of BDNF/TrkB signaling pathway.

关 键 词：牛磺酸 小于胎龄大鼠模型 神经元保护 BDNF/TrkB信号通路 

分 类 号：Q42[生物学—神经生物学] R364.2[生物学—生理学]